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Versatile and facile one-pot biosynthesis for amides and carboxylic acids in E. coli by engineering auxin pathways of plant microbiomes

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Menon, Navya, Richmond, Daniel, Rahman, Mohammad Rejaur and Menon, Binuraj R. K. (2022) Versatile and facile one-pot biosynthesis for amides and carboxylic acids in E. coli by engineering auxin pathways of plant microbiomes. ACS Catalysis, 12 (4). pp. 2309-2319. doi:10.1021/acscatal.1c04901

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Official URL: http://dx.doi.org/10.1021/acscatal.1c04901

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Abstract

The development of enzymatic routes toward amide and carboxylic acid bond formation in bioactive molecular scaffolds using aqueous conditions is a major challenge for biopharmaceutical and fine chemical industrial sectors. We report biocatalytic and kinetic characterization of two indole-3-acetamide (IAM) pathway enzymes, tryptophan-2-monooxygenase (iaaM) and indole-3-acetamide hydrolase (iaaH), present in plant microbiomes that produce indole-3-acetic acid (IAA). In this pathway, tryptophan is converted to indole-3-acetamide by the monooxygenase activity of iaaM, followed by its hydrolysis to form carboxylic acid by iaaH enzyme. Since IAA or auxin is an essential natural plant hormone and an important synthon for fine chemicals, the developed monooxygenase-based bioconversion route has a wider scope compared to currently available synthetic and biocatalytic methods to produce synthetic auxins and a range of amides and carboxylic acids for agrochemical and pharmaceutical applications. To display this, one-pot multienzyme biosynthetic cascades for preparative-scale production of IAA derivatives were performed by incorporating tryptophan synthase and tryptophan halogenase enzymes. We also report the creation of an efficient de novo biosynthesis for IAA and its derivatives from glucose or indoles via a reconstructed IAM pathway in Escherichia coli.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QK Botany
Q Science > QP Physiology
Q Science > QR Microbiology
T Technology > TP Chemical technology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Auxin -- Molecular aspects, Plant-microbe relationships, Synthetic biology, Biosynthesis -- Research, Enzymes, Biocatalysis
Journal or Publication Title: ACS Catalysis
Publisher: American Chemical Society
ISSN: 2155-5435
Official Date: 18 February 2022
Dates:
DateEvent
18 February 2022Published
31 January 2022Available
27 October 2021Submitted
Volume: 12
Number: 4
Page Range: pp. 2309-2319
DOI: 10.1021/acscatal.1c04901
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/M017982/1UK Research and Innovationhttp://dx.doi.org/10.13039/100014013
RGS\R2\180317Royal Societyhttp://dx.doi.org/10.13039/501100000288

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