The Library
Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles
Tools
Malina, Jaroslav, Kostrhunova, Hana, Novohradsky, Vojtech, Scott, Peter and Brabec, Viktor (2022) Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles. Nucleic Acids Research, 50 (2). pp. 674-683. doi:10.1093/nar/gkab1277 ISSN 0305-1048.
|
PDF
WRAP-Metallohelix-vectors-for-efficient-gene-delivery-via-cationic-DNA-nanoparticles-Scott-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons: Attribution-Noncommercial 4.0. Download (4Mb) | Preview |
Official URL: http://dx.doi.org/10.1093/nar/gkab1277
Abstract
The design of efficient and safe gene delivery vehicles remains a major challenge for the application of gene therapy. Of the many reported gene delivery systems, metal complexes with high affinity for nucleic acids are emerging as an attractive option. We have discovered that certain metallohelices—optically pure, self-assembling triple-stranded arrays of fully encapsulated Fe—act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. They induce formation of globular DNA particles which protect the DNA from degradation by various restriction endonucleases, are of suitable size and electrostatic potential for efficient membrane transport and are successfully processed by cells. The activity is highly structure-dependent—compact and shorter metallohelix enantiomers are far less efficient than less compact and longer enantiomers.
Item Type: | Journal Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | T Technology > TA Engineering (General). Civil engineering (General) T Technology > TP Chemical technology |
||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Library of Congress Subject Headings (LCSH): | Gene therapy, Nanoparticles , Metal nanoparticles , DNA nanotechnology | ||||||||||
Journal or Publication Title: | Nucleic Acids Research | ||||||||||
Publisher: | Oxford University Press | ||||||||||
ISSN: | 0305-1048 | ||||||||||
Official Date: | 25 January 2022 | ||||||||||
Dates: |
|
||||||||||
Volume: | 50 | ||||||||||
Number: | 2 | ||||||||||
Page Range: | pp. 674-683 | ||||||||||
DOI: | 10.1093/nar/gkab1277 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Date of first compliant deposit: | 29 March 2022 | ||||||||||
Date of first compliant Open Access: | 1 April 2022 | ||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year