T promoter activity in the absence of functional T protein during axis formation and elongation in the mouse
UNSPECIFIED (1997) T promoter activity in the absence of functional T protein during axis formation and elongation in the mouse. DEVELOPMENTAL BIOLOGY, 189 (2). pp. 161-173. ISSN 0012-1606Full text not available from this repository.
The T (Brachyury) gene, which encodes a transcription factor, is involved in the formation of posterior mesoderm. Although studies in Xenopus have shown that T gene expression is responsive to mesoderm inducing factors and furthermore suggest the existence of an autoregulatory loop involving T itself, eFGF, and the EGF receptor, little is known about the regulation of T expression in the mouse. We report here that in the mouse the expression of fgf-4, the putative homologue of Xenopus efgf, is not dependent on the presence of T protein during the first 48 hr of gastrulation. Furthermore, we address the question of autoregulation using a chimeric approach. Introduction of a T promoter-lacZ construct into TIT ES cells results in T promoter activity in the primitive streak and tail bud in the absence of functional T protein. Therefore, we suggest that a direct FGF and T autoregulatory loop is unlikely to operate during gastrulation and axis elongation in the mouse. (C) 1997 Academic Press.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Journal or Publication Title:||DEVELOPMENTAL BIOLOGY|
|Publisher:||ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS|
|Date:||15 September 1997|
|Number of Pages:||13|
|Page Range:||pp. 161-173|
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