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The plant endoplasmic reticulum is both receptive and responsive to pathogen effectors
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Breeze, Emily, Vale, Victoria, McLellan, Hazel, Godiard, Laurence, Grant, Murray and Frigerio, Lorenzo (2020) The plant endoplasmic reticulum is both receptive and responsive to pathogen effectors. Working Paper. New York: Cold Spring Harbor Laboratory.
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Official URL: https://doi.org/10.1101/2020.06.09.142141
Abstract
The endoplasmic reticulum (ER) is the entry point to the secretory pathway and, as such, is critical for adaptive responses to biotic stress, when the demand for de novo synthesis of immunity-related proteins and signalling components increases significantly. Comprised of a network of interconnected tubules and cisternae, the architecture of the ER is highly pleomorphic and dynamic, rapidly remodelling to meet new cellular requirements. During infection with the hemi-biotrophic phytopathogen, Pseudomonas syringae pv. tomato DC3000, the ER in cells immediately adjacent to established bacterial colonies condenses into ‘knot-like’ structures, reminiscent of fenestrated sheets. Based on known temporal dynamics of pathogen effector delivery and initial bacterial multiplication, the timing of these observed morphological changes is rapid and independent of classical elicitor activation of pathogen-triggered immunity. To further investigate a role for ER reconfiguration in suppression of plant immunity we identified a conserved C-terminal tail-anchor domain in a set of pathogen effectors known to localize to the ER and used this protein topology in an in silico screen to identify putative ER-localised effectors within the effectorome of the oomycete Phytophthora infestans. Subsequent characterization of a subset of 15 candidate tail- anchored P. infestans effectors revealed that 11 localised to the ER and/or Golgi. Notably, transient expression of an ER-localised effector from the closely related oomycete, Plasmopara halstedii, reconfigured the ER network, revealing intimate association of labelled ER with perinuclear chloroplasts and clusters of chloroplasts, potentially facilitating retrograde signalling during plant defence.
| Item Type: | Working or Discussion Paper (Working Paper) | |||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QK Botany |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | |||||||||
| Library of Congress Subject Headings (LCSH): | Endoplasmic reticulum, Endoplasmic reticulum -- Pathophysiology , Plant physiology , Plant cell membranes, Plants -- Effect of stress on | |||||||||
| Journal or Publication Title: | bioRxiv | |||||||||
| Publisher: | Cold Spring Harbor Laboratory | |||||||||
| Place of Publication: | New York | |||||||||
| Official Date: | 11 June 2020 | |||||||||
| Dates: |
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| Number of Pages: | 32 | |||||||||
| DOI: | 10.1101/2020.06.09.142141 | |||||||||
| Institution: | University of Warwick | |||||||||
| Status: | Not Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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