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Spinach-based RNA mimicking GFP in plant cells
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Yu, Zhiming, Wang, Yue, Mei, Fengling, Yan, Haiting, Jin, Zhenhui, Zhang, Pengcheng, Zhang, Xian, Tör, Mahmut, Jackson, Stephen D. , Shi, Nongnong and Hong, Yiguo (2022) Spinach-based RNA mimicking GFP in plant cells. Functional & Integrative Genomics, 22 . pp. 423-428. doi:10.1007/s10142-022-00835-x ISSN 1438-793X.
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WRAP-spinach-based-RNA-mimicking-GFP-plant-cells-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (864Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s10142-022-00835-x
Abstract
Spinach RNA-mimicking GFP (S-RMG) has been successfully used to monitor cellular RNAs including microRNAs in bacterium, yeast, and human cells. However, S-RMG has not been established in plants. In this study, we found that like bacterial, yeast, and human cellular tRNAs, plant tRNAs such as tRNALys can protect and/or stabilize the Spinach RNA aptamer interaction with the fluorophore DFHBI enabling detectable levels of green fluorescence to be emitted. The tRNALys-Spinach-tRNALys, once delivered into “chloroplast-free” onion epidermal cells can emit strong green fluorescence in the presence of DFHBI. Our results demonstrate for the first time that Spinach-based RNA visualization has the potential for in vivo monitoring of RNAs in plant cells.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QK Botany | ||||||||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Plant physiology -- Genetic aspects, Plant morphology, RNA, Nucleic acids, Gene expression -- Research, Plant cell walls, Fluorescence, Spinach | ||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Functional & Integrative Genomics | ||||||||||||||||||||||||||||||||||||
| Publisher: | Springer | ||||||||||||||||||||||||||||||||||||
| ISSN: | 1438-793X | ||||||||||||||||||||||||||||||||||||
| Official Date: | June 2022 | ||||||||||||||||||||||||||||||||||||
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| Volume: | 22 | ||||||||||||||||||||||||||||||||||||
| Page Range: | pp. 423-428 | ||||||||||||||||||||||||||||||||||||
| DOI: | 10.1007/s10142-022-00835-x | ||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 17 March 2022 | ||||||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 21 March 2022 | ||||||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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