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Persistent parental RNAi in the Beetle Tribolium castaneum involves maternal transmission of long double‐stranded RNA

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Horn, Thorsten, Narov, Kalin and Panfilio, Kristen A. (2022) Persistent parental RNAi in the Beetle Tribolium castaneum involves maternal transmission of long double‐stranded RNA. Advanced Genetics . 2100064. doi:10.1002/ggn2.202100064

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Official URL: https://doi.org/10.1002/ggn2.202100064

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Abstract

Parental RNA interference (pRNAi) is a powerful and widely used method for gene‐specific knockdown. Yet in insects its efficacy varies between species, and how the systemic response is transmitted from mother to offspring remains elusive. Using the beetle Tribolium castaneum, an RT‐qPCR strategy to distinguish the presence of double‐stranded RNA (dsRNA) from endogenous mRNA is reported. It is found that injected dsRNA is directly transmitted into the egg and persists throughout embryogenesis. Despite this depletion of dsRNA from the mother, it is shown that strong pRNAi can persist for months before waning at strain‐specific rates. In seeking the receptor proteins for cellular uptake of long dsRNA into the egg, a phylogenomics profiling approach of candidate proteins is also presented. A visualization strategy based on taxonomically hierarchical assessment of orthology clustering data to rapidly assess gene age and copy number changes, refined by sequence‐based evidence, is demonstrated. Repeated losses of SID‐1‐like channel proteins in the arthropods, including wholesale loss in the Heteroptera (true bugs), which are nonetheless highly sensitive to pRNAi, are thereby documented. Overall, practical considerations for insect pRNAi against a backdrop of outstanding questions on the molecular mechanism of dsRNA transmission for long‐term, systemic knockdown are elucidated.

Item Type: Journal Article
Subjects: Q Science > QL Zoology
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): RNA , Red flour beetle -- Phylogeny, Double-stranded RNA, Genetics
Journal or Publication Title: Advanced Genetics
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 2641-6573
Official Date: 2022
Dates:
DateEvent
2022Published
20 March 2022Available
1 February 2022Accepted
Article Number: 2100064
DOI: 10.1002/ggn2.202100064
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
PA2044/1-1[DFG] Deutsche Forschungsgemeinschafthttp://dx.doi.org/10.13039/501100001659
BB/V002392/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/V002392/1UK Research and Innovationhttp://dx.doi.org/10.13039/100014013
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