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Inexpensive protein overexpression driven by the NarL transcription activator protein
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Hothersall, Joanne, Lai, Sandie, Zhang, Nana, Godfrey, Rita E., Ruanto, Patcharawarin, Bischoff, Sarah, Robinson, Colin, Overton, Tim W., Busby, Stephen J. W. and Browning, Douglas F. (2022) Inexpensive protein overexpression driven by the NarL transcription activator protein. Biotechnology and Bioengineering, 119 (6). pp. 1614-1623. doi:10.1002/bit.28071
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WRAP-inexpensive-protein-overexpression-driven-NarL-transcription-activator-protein-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2270Kb) | Preview |
Official URL: http://dx.doi.org/10.1002/bit.28071
Abstract
Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple conjugates. However, these can be expensive and, sometimes, chemically unstable. To simplify this and to cut the cost of RPP, we have developed vectors controlled by the Escherichia coli nitrate-responsive NarL transcription activator protein, which use nitrate, a cheap, stable, and abundant inorganic ion, to induce high-level controlled RPP. We show that target proteins, such as green fluorescent protein, human growth hormone, and single-chain variable region antibody fragments can be expressed to high levels using our promoter systems. As nitrate levels are high in many commercial fertilizers, we demonstrate that controlled RPP can be achieved using readily available and inexpensive garden products.
| Item Type: | Journal Article | ||||||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology T Technology > TP Chemical technology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Biopharmaceutics, Escherichia coli, Recombinant proteins, Genetic transcription -- Regulation | ||||||||||||||||||
| Journal or Publication Title: | Biotechnology and Bioengineering | ||||||||||||||||||
| Publisher: | John Wiley & Sons, Inc. | ||||||||||||||||||
| ISSN: | 0006-3592 | ||||||||||||||||||
| Official Date: | June 2022 | ||||||||||||||||||
| Dates: |
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| Volume: | 119 | ||||||||||||||||||
| Number: | 6 | ||||||||||||||||||
| Page Range: | pp. 1614-1623 | ||||||||||||||||||
| DOI: | 10.1002/bit.28071 | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
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