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3D localisation of mosquitoes using digital holography over an extended field of view
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Hall, Matthew L. (2021) 3D localisation of mosquitoes using digital holography over an extended field of view. PhD thesis, University of Warwick.
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WRAP_Theses_Hall_2021.pdf - Submitted Version - Requires a PDF viewer. Download (35Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3755562
Abstract
Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria transmission. Accurate 3D time-series imaging of mosquito-bednet interaction has not previously been possible, but has the potential to offer full kinematic analysis of mosquito flight, accurately determine in situ insecticide dosages, and examine behaviours not yet welldocumented. The work presented in this thesis relates to utilising digital holography for the accurate 3D imaging of mosquito flight using a single-view recording setup. A scalable digital methodology for single object reconstruction and localisation is developed through simulations and physical validation, and a novel focus metric, based on the zero-crossing of the reconstructed complex wavefront, is introduced. This work examines the boundaries presented by a digital hologram recording setup, and utilises the lowest imaging resolution that still yields acceptable localisation errors. Real-world problems typical in mosquito imaging, such as multi-object close proximity and occlusions, and obscured-object localisation, are examined in detail. The methods provided to overcome these obstacles largely involve the development of novel post-process software solutions. Finally, a large field of view digital holographic experiment is developed and preliminary qualitative data of livemosquito reconstruction and localisation is presented. Further work involving techniques to improved the calibration of the large field of view system are suggested to improve the quantitative analysis of the data. The result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D planar imaging of mosquitoes in a much smaller volume, as well as introducing novel post-processing techniques for accurate localisation.
| Item Type: | Thesis (PhD) | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QC Physics Q Science > QL Zoology T Technology > TA Engineering (General). Civil engineering (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering |
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| Library of Congress Subject Headings (LCSH): | Holography, Three-dimensional imaging, Image processing -- Digital techniques, Mosquitoes -- Flight, Mosquitoes -- Behavior | ||||
| Official Date: | July 2021 | ||||
| Dates: |
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| Institution: | University of Warwick | ||||
| Theses Department: | School of Engineering | ||||
| Thesis Type: | PhD | ||||
| Publication Status: | Unpublished | ||||
| Supervisor(s)/Advisor: | Towers, Catherine E. (Catherine Elizabeth) ; Towers, David A. | ||||
| Format of File: | |||||
| Extent: | xxii, 190 leaves : illustrations | ||||
| Language: | eng |
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