Bennett, Amanda J., Mead, A. (Andrew) and Whipps, J. M.. (2009) Performance of carrot and onion seed primed with beneficial microorganisms in glasshouse and field trials. Biological Control, Vol.51 (No.3). pp. 417-426. ISSN 1049-9644

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Abstract

Beneficial microorganisms (Clonostachys rosea IK726, Pseudomonas chlororaphis MA342, Pseudomonas fluorescens CHA0, Trichoderma harzianum T22 and Trichoderma viride S17a) were successfully applied to carrot and onion seed during a commercial drum priming process. Applied microorganisms were recovered above the target of at least 1 × 105 cfu g−1 seed following subsequent application of pesticides to the seed according to standard commercial practices of film-coating carrot and pelletting onion seed. Two glasshouse experiments consistently showed that priming improved emergence of carrot seed and that C. rosea IK726 further improved emergence time. Priming improved emergence of onion seed in one glasshouse experiment, but had an unexpected negative effect on emergence in the second experiment, possibly due to the proliferation of an unidentified indigenous microorganism during priming, becoming deleterious in high numbers. In this experiment, the application of beneficial microorganisms during priming negated this effect and significantly improved emergence. For each crop, a series of field trials was also carried out over three years, at two different sites each year. Although some positive effects of different seed treatments were seen on emergence or yield in individual field trials, no consistent effects were found for primed or microorganism-treated seed across all sites and years. However, a combined analysis of data for all years and sites indicated that pesticide application did consistently improve emergence and yield for both carrot and onion. This is the first comprehensive study assessing glasshouse and field performance of carrot and onion seed primed with beneficial microorganisms during a commercial process of drum priming in the UK.

Item Type:	Journal Article
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Gliocladium -- Biotechnology, Pseudomonas -- Biotechnology, Trichoderma -- Biotechnology, Seed adulteration and inspection, Onions -- Seeds, Carrots -- Seeds, Crop yields
Journal or Publication Title:	Biological Control
Publisher:	Elsevier
ISSN:	1049-9644
Date:	December 2009
Volume:	Vol.51
Number:	No.3
Page Range:	pp. 417-426
Identification Number:	10.1016/j.biocontrol.2009.08.001
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), Great Britain. Ministry of Agriculture, Fisheries and Food (MAFF), Elsoms Seeds Ltd. (ES), Germain Seed and Plant Company (GSPC), Horticultural Development Council (Great Britain) (HDC)
References:	Adams, 1990 P.B. Adams, The potential of mycoparasites for biological control of plant diseases, Annual Review of Phytopathology 28 (1990), pp. 59–72. Barazani and Friedman, 2001 O. Barazani and J. Friedman, Allelopathic bacteria and their impact on higher plants, Critical Reviews in Microbiology 27 (2001), pp. 41–55. Bennett and Whipps, 2008a A.J. Bennett and J.M. Whipps, Beneficial microorganism survival on seed, roots and in rhizosphere soil following application to seed during drum priming, Biological Control 44 (2008), pp. 349–361. Bennett and Whipps, 2008b A.J. Bennett and J.M. Whipps, Dual application of beneficial microorganisms to seed during drum priming, Applied Soil Ecology 38 (2008), pp. 83–89. Callan et al., 1991 N.W. Callan, D.E. Mathre and J.B. Miller, Field performance of sweet corn seed bio-primed and coated with Pseudomonas fluorescens AB254, HortScience 26 (1991), pp. 1163–1165. Clarkson et al., 2002 J.P. Clarkson, T. Payne, A. Mead and J.M. Whipps, Selection of fungal biological control agents of Sclerotium cepivorum for control of white rot by sclerotial degradation in a UK soil, Plant Pathology 51 (2002), pp. 735–745. Clarkson et al., 2006 J.P. Clarkson, A. Scruby, A. Mead, C. Wright, B. Smith and J.M. Whipps, Integrated control of Allium white rot with Trichoderma viride, tebuconazole and composted onion waste, Plant Pathology 55 (2006), pp. 375–386. El-Mougy and Abdel-Kader, 2008 N.S. El-Mougy and M.M. Abdel-Kader, Long-term activity of bio-priming seed treatment for biological control of faba bean root rot pathogens, Australasian Plant Pathology 37 (2008), pp. 464–471. Fravel et al., 1998 D.R. Fravel, W.J. Connick Jr. and J.A. Lewis, Formulation of microorganisms to control plant diseases. In: H.D. Burges, Editor, Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments, Kluwer Academic Publishers, Dordrecht (1998), pp. 187–202. Gerhardson, 2002 B. Gerhardson, Biological substitutes for pesticides, Trends in Biotechnology 20 (2002), pp. 338–343. Giri and Schillinger, 2003 G.S. Giri and W.F. Schillinger, Seed priming winter wheat for germination, emergence, and yield, Crop Science 43 (2003), pp. 2135–2141. Halmer, 2004 P. Halmer, Methods to improve seed performance in the field. In: R.L. Benech-Arnold and R.A. Sánchez, Editors, Handbook of Seed Physiology: Applications to Agriculture, The Haworth Press, Inc., New York (2004), pp. 125–166. Harman, 1991 G.E. Harman, Seed treatments for biological control of plant disease, Crop Protection 10 (1991), pp. 166–171. Harman et al., 1989 G.E. Harman, A.G. Taylor and T.E. Stasz, Combining effective strains of Trichoderma harzianum and solid matrix priming to improve biological seed treatments, Plant Disease 73 (1989), pp. 631–637. Jensen et al., 2002 B. Jensen, I.M.B. Knudsen and D.F. Jensen, Survival of conidia of Clonostachys rosea on stored barley seeds and their biocontrol efficacy against seed-borne Bipolaris sorokiniana, Biocontrol Science and Technology 12 (2002), pp. 427–441. Jensen et al., 2004 B. Jensen, I.M.B. Knudsen, M. Madsen and D.F. Jensen, Biopriming of infected carrot seed with an antagonist, Clonostachys rosea, selected for control of seedborne Alternaria spp., Phytopathology 94 (2004), pp. 551–560. Jensen et al., 2007 D.F. Jensen, I.M.B. Knudsen, M. Lübeck, M. Mamarabadi, J. Hockenhull and B. Jensen, Development of a biocontrol agent for plant disease control with special emphasis on the near commercial fungal antagonist Clonostachys rosea strain ‘IK726’, Australasian Plant Pathology 36 (2007), pp. 95–101. Johnsson et al., 1998 L. Johnsson, M. Hökeberg and B. Gerhardson, Performance of the Pseudomonas chlororaphis biocontrol agent MA 342 against cereal seed-borne diseases in field experiments, European Journal of Plant Pathology 104 (1998), pp. 701–711. Mathre et al., 1999 D.E. Mathre, R.J. Cook and N.W. Callan, From discovery to use: Traversing the world of commercializing biocontrol agents for plant disease control, Plant Disease 83 (1999), pp. 972–983. Maurhofer et al., 1992 M. Maurhofer, C. Keel, U. Schnider, C. Voisard, D. Haas and G. Défago, Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHA0 on its disease suppressive capacity, Phytopathology 82 (1992), pp. 190–195. Maurhofer et al., 1994 M. Maurhofer, P. Sacherer, C. Keel, D. Haas, G. Défago, M.H. Ryder, P.M. Stephens and G.D. Bowen, Role of some metabolites produced by Pseudomonas fluorescens CHA0 in the suppression of different plant diseases. In: M.H. Ryder, P.M. Stephens and G.D. Bowen, Editors, Improving Plant Productivity with Rhizosphere Bacteria: Proceedings of the Third International Workshop on Plant Growth-Promoting Rhizobacteria, CSIRO, Adelaide (1994), pp. 117–119. McQuilken et al., 1998 M.P. McQuilken, P. Halmer and D.J. Rhodes, Application of microorganisms to seeds. In: H.D. Burges, Editor, Formulation of Microbial Biopesticides: Beneficial microorganisms, Nematodes and Seed Treatments, Kluwer Academic Publishers, Dordrecht (1998), pp. 255–285. Nascimento and West, 1998 W.M. Nascimento and S.H. West, Microorganism growth during muskmelon seed priming, Seed Science and Technology 26 (1998), pp. 531–534. Niranjan et al., 2004 S. Niranjan, N.P. Shetty and H.S. Shetty, Seed bio-priming with Pseudomonas fluorescens isolates enhances growth of pearl millet plants and induces resistance against downy mildew, International Journal of Pest Management 50 (2004), pp. 41–48. Olszewski et al., 2005 M.W. Olszewski, T.A. Evans, N.F. Gregory and W.G. Pill, Enhanced germination of primed mericarps of parsley (Petroselinum crispum Mill. Nyman ex A.W. Hill) limited by Altemaria alternata proliferation, Journal of Horticultural Science and Biotechnology 80 (2005), pp. 427–432. Raaimakers and Weller, 1998 J.M. Raaimakers and D.M. Weller, Natural plant protection by 2, 4-diacetylphloroglucinol-producing Pseudomonas spp. in take-all decline soils, Molecular Plant-Microbe Interactions 11 (1998), pp. 144–152. Ravnskov et al., 2006 S. Ravnskov, B. Jensen, I.M.B. Knudsen, L. Bødker, D. Funck Jensen, L. Karlinski and J. Larsen, Soil inoculation with the biocontrol agent Clonostachys rosea and the mycorrhizal fungus Glomus intraradices results in mutual inhibition, plant growth promotion and alteration of soil microbial communities, Soil Biology and Biochemistry 38 (2006), pp. 3453–3462. Rowse, 1996a H.R. Rowse, Drum priming—a non-osmotic method of priming seeds, Seed Science and Technology 24 (1996), pp. 281–294. Rowse, 1996b H.R. Rowse, Drum priming—an environmentally-friendly way of improving seed performance, Journal of the Royal Agricultural Society of England 157 (1996), pp. 77–83. Subedi and Ma, 2005 K.D. Subedi and B.L. Ma, Seed priming does not improve corn yield in a humid temperate environment, Agronomy Journal 97 (2005), pp. 211–218. Tylkowska and van den Bulk, 2001 K. Tylkowska and R.W. van den Bulk, Effects of osmo- and hydropriming on fungal infestation levels and germination of carrot (Daucus carota L.) seeds contaminated with Alternaria spp., Seed Science and Technology 29 (2001), pp. 365–375. Walker et al., 2004 R. Walker, S. Rossall and M.J.C. Asher, Comparison of application methods to prolong the survival of potential biocontrol bacteria on stored sugar-beet seed, Journal of Applied Microbiology 97 (2004), pp. 293–305. Warren and Bennett, 2000 J.E. Warren and M.A. Bennett, Bio-osmopriming tomato (Lycopersicon esculentum Mill.) seeds for improved seedling establishment. In: M. Black, K.J. Bradford and J. Vásquez-Ramos, Editors, Seed Biology: Advances and Applications, CAB International, pp (2000), pp. 477–487. Whipps, 2001 J.M. Whipps, Microbial interactions and biocontrol in the rhizosphere, Journal of Experimental Botany 52 (2001), pp. 487–511. Wright et al., 2003a B. Wright, H. Rowse and J.M. Whipps, Microbial population dynamics on seeds during drum and steeping priming, Plant and Soil 255 (2003), pp. 631–640. Wright et al., 2003b B. Wright, H.R. Rowse and J.M. Whipps, Application of beneficial microorganisms to seeds during drum priming, Biocontrol Science and Technology 13 (2003), pp. 599–614.
URI:	http://wrap.warwick.ac.uk/id/eprint/1645

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