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Measuring intercellular interface area in plant tissues using quantitative 3D image analysis
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Davis, Gwendolyn V., Smith, Richard S. and Bassel, George (2022) Measuring intercellular interface area in plant tissues using quantitative 3D image analysis. In: Benitez-Alfonso, Y. and Heinlein , M., (eds.) Plasmodesmata. Methods in Molecular Biology, 2457 . Humana Press: New York, NY, pp. 457-464. ISBN 9781071621318
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Official URL: https://doi.org/10.1007/978-1-0716-2132-5_31
Abstract
The cells which make up plant tissues remain fixed together through shared cell walls. Cell-to-cell communication principally takes place through these shared interfaces through a combination of plasmodesmata, transporters, and the apoplastic space. To better understand the capacity for intercellular communication in plant tissues, this chapter outlines a method which can be used to quantify the surface area of shared intercellular interfaces using whole mount imaging and quantitative 3D image analysis. This method allows the potential for intercellular communication as prescribed by cellular architecture to be measured at single cell resolution. [Abstract copyright: © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.]
Item Type: | Book Item | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
SWORD Depositor: | Library Publications Router | ||||
Series Name: | Methods in Molecular Biology | ||||
Journal or Publication Title: | Methods in Molecular Biology | ||||
Publisher: | New York, NY | ||||
Place of Publication: | Humana Press | ||||
ISBN: | 9781071621318 | ||||
ISSN: | 1064-3745 | ||||
Book Title: | Plasmodesmata | ||||
Editor: | Benitez-Alfonso, Y. and Heinlein , M. | ||||
Official Date: | 30 March 2022 | ||||
Dates: |
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Volume: | 2457 | ||||
Page Range: | pp. 457-464 | ||||
DOI: | 10.1007/978-1-0716-2132-5_31 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Copyright Holders: | © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022 | ||||
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