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The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro
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Zhu, Jiajing, Tian, Yanling, Yang, Jing, Hu, Jing, Wang, Zuobin and Liu, Xianping (2022) The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro. Journal of Microscopy, 287 (1). pp. 3-18. doi:10.1111/jmi.13104 ISSN 0022-2720.
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Official URL: https://doi.org/10.1111/jmi.13104
Abstract
Cancer is now responsible for the major leading cause of death worldwide. It is noteworthy that lung cancer has been recognised as the highest incidence (11.6%) and mortality (18.4%) for combined sexes among a variety of cancer diseases. Therefore, it is of great value to investigate the mechanical properties of lung cancerous cells for early diagnosis. This paper focus on the influence of measurement parameters on the measured central Young's moduli of single live A549 cell in vitro based on the force spectroscopy mode of atomic force microscopy (AFM). The effects of the measurement parameters on the measured central Young's moduli were analysed by fitting the force–depth curves utilising the Sneddon model. The results revealed that the Young's moduli of A549 cells increased with the larger indentation force, higher indentation speed, less retraction time, deeper Z length and lower purity percentage of serum. The Young's moduli of cells increased first and then decreased with the increasing dwell time. Hence, this research may have potential significance to provide reference for the standardised detection of a single cancerous cell in vitro using AFM methodologies.
| Item Type: | Journal Article | |||||||||||||||||||||||||||
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| Subjects: | Q Science > QM Human anatomy Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | |||||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Epithelial cells, Atomic force microscopy , Cells -- Mechanical properties , Cells -- Morphology | |||||||||||||||||||||||||||
| Journal or Publication Title: | Journal of Microscopy | |||||||||||||||||||||||||||
| Publisher: | Wiley-Blackwell Publishing, Inc | |||||||||||||||||||||||||||
| ISSN: | 0022-2720 | |||||||||||||||||||||||||||
| Official Date: | July 2022 | |||||||||||||||||||||||||||
| Dates: |
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| Volume: | 287 | |||||||||||||||||||||||||||
| Number: | 1 | |||||||||||||||||||||||||||
| Page Range: | pp. 3-18 | |||||||||||||||||||||||||||
| DOI: | 10.1111/jmi.13104 | |||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
| Date of first compliant deposit: | 25 May 2022 | |||||||||||||||||||||||||||
| Date of first compliant Open Access: | 26 May 2022 | |||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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