UNSPECIFIED (1997) Copper-dependent reciprocal transcriptional regulation of methane monooxygenase genes in Methylococcus capsulatus and Methylosinus trichosporium. MOLECULAR MICROBIOLOGY, 25 (2). pp. 399-409. ISSN 0950-382X

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Abstract

The methanotrophic bacteria Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b convert methane to methanol using the enzyme, methane monooxygenase (MMO). These bacteria are able to express two distinct MMOs: a cytoplasmic or soluble form (sMMO) and a membrane-bound or particulate form (pMMO). Differential expression of sMMO and pMMO is regulated by the amount of copper ions available to the cells; sMMO is expressed at low copper-biomass ratios, whereas pMMO is expressed at high copper-biomass ratios. In both methanotrophs, transcription of the sMMO gene cluster is negatively regulated by copper ions. Data suggest that transcription of the M. trichosporium OB3b sMMO gene cluster is directed from a sigma(54)-like and a sigma(70)-like promoter. The pMMO (pmo) genes of M. capsulatus (Bath) are transcribed into a polycistronic mRNA of 3.3 kb. The synthesis of this mRNA was activated by copper ions. Activation of pmo transcription by copper ions was concomitant with repression of sMMO gene transcription in both methanotrophs. This suggests that a common regulatory pathway may be involved in the transcriptional switch between sMMO and pMMO gene expression.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Journal or Publication Title:	MOLECULAR MICROBIOLOGY
Publisher:	BLACKWELL SCIENCE LTD
ISSN:	0950-382X
Date:	July 1997
Volume:	25
Number:	2
Number of Pages:	11
Page Range:	pp. 399-409
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/16491

Data sourced from Thomson Reuters' Web of Knowledge

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