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Bottlebrush copolymers for gene delivery : influence of architecture, charge density, and backbone length on transfection efficiency

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Floyd, Thomas G., Song, Ji-Inn, Hapeshi, Alexia, Laroque, Sophie, Hartlieb, Matthias and Perrier, Sébastien (2022) Bottlebrush copolymers for gene delivery : influence of architecture, charge density, and backbone length on transfection efficiency. Journal of Materials Chemistry B, 10 (19). pp. 3696-3704. doi:10.1039/d2tb00490a ISSN 2050-750X.

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Official URL: https://doi.org/10.1039/d2tb00490a

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Abstract

The influence of polymer architecture of polycations on their ability to transfect mammalian cells is probed. Polymer bottle brushes with grafts made from partially hydrolysed poly(2-ethyl-2-oxazoline) are used while varying the length of the polymer backbone as well as the degree of hydrolysis (cationic charge content). Polyplex formation is investigated via gel electrophoresis, dye-displacement and dynamic light scattering. Bottle brushes show a superior ability to complex pDNA when compared to linear copolymers. Also, nucleic acid release was found to be improved by a graft architecture. Polyplexes based on bottle brush copolymers showed an elongated shape in transmission electron microscopy images. The cytotoxicity against mammalian cells is drastically reduced when a graft architecture is used instead of linear copolymers. Moreover, the best-performing bottle brush copolymer showed a transfection ability comparable with that of linear poly(ethylenimine), the gold standard of polymeric transfection agents, which is used as positive control. In combination with their markedly lowered cytotoxicity, cationic bottle brush copolymers are therefore shown to be a highly promising class of gene delivery vectors.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
T Technology > TP Chemical technology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Copolymers , Graft copolymers , Animal cell biotechnology, Gene expression
Journal or Publication Title: Journal of Materials Chemistry B
Publisher: R S C Publications
ISSN: 2050-750X
Official Date: 21 May 2022
Dates:
DateEvent
21 May 2022Published
11 April 2022Available
11 April 2022Accepted
Volume: 10
Number: 19
Page Range: pp. 3696-3704
DOI: 10.1039/d2tb00490a
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 9 June 2022
Date of first compliant Open Access: 11 April 2023
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
HA 7725/2-1[DFG] Deutsche Forschungsgemeinschafthttp://dx.doi.org/10.13039/501100001659
MC_PC_17136Medical Research Councilhttp://dx.doi.org/10.13039/501100000265

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