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Bottlebrush copolymers for gene delivery : influence of architecture, charge density, and backbone length on transfection efficiency
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Floyd, Thomas G., Song, Ji-Inn, Hapeshi, Alexia, Laroque, Sophie, Hartlieb, Matthias and Perrier, Sébastien (2022) Bottlebrush copolymers for gene delivery : influence of architecture, charge density, and backbone length on transfection efficiency. Journal of Materials Chemistry B, 10 (19). pp. 3696-3704. doi:10.1039/d2tb00490a ISSN 2050-750X.
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WRAP-Bottlebrush-copolymers-gene-delivery-architecture-backbone-transfection-efficiency-2022.pdf - Accepted Version - Requires a PDF viewer. Download (3379Kb) | Preview |
Official URL: https://doi.org/10.1039/d2tb00490a
Abstract
The influence of polymer architecture of polycations on their ability to transfect mammalian cells is probed. Polymer bottle brushes with grafts made from partially hydrolysed poly(2-ethyl-2-oxazoline) are used while varying the length of the polymer backbone as well as the degree of hydrolysis (cationic charge content). Polyplex formation is investigated via gel electrophoresis, dye-displacement and dynamic light scattering. Bottle brushes show a superior ability to complex pDNA when compared to linear copolymers. Also, nucleic acid release was found to be improved by a graft architecture. Polyplexes based on bottle brush copolymers showed an elongated shape in transmission electron microscopy images. The cytotoxicity against mammalian cells is drastically reduced when a graft architecture is used instead of linear copolymers. Moreover, the best-performing bottle brush copolymer showed a transfection ability comparable with that of linear poly(ethylenimine), the gold standard of polymeric transfection agents, which is used as positive control. In combination with their markedly lowered cytotoxicity, cationic bottle brush copolymers are therefore shown to be a highly promising class of gene delivery vectors.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history T Technology > TP Chemical technology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Copolymers , Graft copolymers , Animal cell biotechnology, Gene expression | |||||||||
| Journal or Publication Title: | Journal of Materials Chemistry B | |||||||||
| Publisher: | R S C Publications | |||||||||
| ISSN: | 2050-750X | |||||||||
| Official Date: | 21 May 2022 | |||||||||
| Dates: |
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| Volume: | 10 | |||||||||
| Number: | 19 | |||||||||
| Page Range: | pp. 3696-3704 | |||||||||
| DOI: | 10.1039/d2tb00490a | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||
| Date of first compliant deposit: | 9 June 2022 | |||||||||
| Date of first compliant Open Access: | 11 April 2023 | |||||||||
| RIOXX Funder/Project Grant: |
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