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The proteobacterial methanotroph Methylosinus trichosporium OB3b remodels membrane lipids in response to phosphate limitation
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Scanlan, Julie, Guillonneau, Richard, Cunningham, Mark R., Najmin, Sahanara, Mausz, Michaela A., Murphy, Andrew, Murray, Leanne L., Zhang, Limei, Kumaresan, Deepak and Chen, Yin (2022) The proteobacterial methanotroph Methylosinus trichosporium OB3b remodels membrane lipids in response to phosphate limitation. mBio, 13 (3). e00247-22. doi:10.1128/mbio.00247-22 ISSN 2150-7511.
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Official URL: https://doi.org/10.1128/mbio.00247-22
Abstract
Methane is a potent greenhouse gas in the atmosphere, and its concentration has continued to increase in recent decades. Aerobic methanotrophs, bacteria that use methane as the sole carbon source, are an important biological sink for methane, and they are widely distributed in the natural environment. However, relatively little is known on how methanotroph activity is regulated by nutrients, particularly phosphorus (P). P is the principal nutrient constraining plant and microbial productivity in many ecosystems, ranging from agricultural land to the open ocean. Using a model methanotrophic bacterium, Methylosinus trichosporium OB3b, we demonstrate here that this bacterium can produce P-free glycolipids to replace membrane phospholipids in response to P limitation. The formation of the glycolipid monoglucuronic acid diacylglycerol requires plcP-agt genes since the plcP-agt mutant is unable to produce this glycolipid. This plcP-agt-mediated lipid remodeling pathway appears to be important for M. trichosporium OB3b to cope with P stress, and the mutant grew significantly slower under P limitation. Interestingly, comparative genomics analysis shows that the ability to perform lipid remodeling appears to be a conserved trait in proteobacterial methanotrophs; indeed, plcP is found in all proteobacterial methanotroph genomes, and plcP transcripts from methanotrophs are readily detectable in metatranscriptomics data sets. Together, our study provides new insights into the adaptation to P limitation in this ecologically important group of bacteria.
| Item Type: | Journal Article | ||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Journal or Publication Title: | mBio | ||||||||
| Publisher: | American Society for Microbiology | ||||||||
| ISSN: | 2150-7511 | ||||||||
| Official Date: | June 2022 | ||||||||
| Dates: |
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| Volume: | 13 | ||||||||
| Number: | 3 | ||||||||
| Number of Pages: | 14 | ||||||||
| Article Number: | e00247-22 | ||||||||
| DOI: | 10.1128/mbio.00247-22 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 29 April 2022 | ||||||||
| Date of first compliant Open Access: | 16 May 2022 |
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