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Xenopus Wnt11b is identified as a potential pronephric inducer
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Tételin, Stéphanie and Jones, Elizabeth A.. (2010) Xenopus Wnt11b is identified as a potential pronephric inducer. Developmental Dynamics, Vol.239 (Vol.1 Special Issue). pp. 148-159. ISSN 1058-8388
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Official URL: http://dx.doi.org/10.1002/dvdy.22012
Abstract
In this study, we aimed to establish if known wnt signaling molecules could be responsible for inducing early pronephros specification, using a novel and effective in vitro bioassay in Xenopus embryos. Anterior somites have the unique biological activity to signal to unspecified intermediate mesoderm to induce pronephros formation in Xenopus embryos. We have used a molecular candidate gene approach to analyze both canonical and noncanonical wnt expression in isolated anterior and posterior somites and dissected presumptive pronephros, pronephric anlagen, and pronephros from stage 12.5-35 embryos. We have identified potential candidate wnt genes expressed in the right time and place to specify pronephric development. These candidates were then directly tested in an in vitro pronephrogenesis assay based on Holtfreter sandwich cultures. Results revealed that noncanonical wnt11b and wnt11 can induce pronephros formation in vitro. Loss-of-function experiments confirmed that these genes are necessary for normal pronephros development. Developmental Dynamics 239:148-159, 2010. (C) 2009 Wiley-Liss, Inc.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QL Zoology Q Science > QH Natural history > QH301 Biology |
| Journal or Publication Title: | Developmental Dynamics |
| Publisher: | John Wiley & Sons Ltd. |
| ISSN: | 1058-8388 |
| Date: | January 2010 |
| Volume: | Vol.239 |
| Number: | Vol.1 Special Issue |
| Number of Pages: | 12 |
| Page Range: | pp. 148-159 |
| Identification Number: | 10.1002/dvdy.22012 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Warwick University |
| Grant number: | G1988, G12713 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/16596 |
Data sourced from Thomson Reuters' Web of Knowledge
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