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Multimodal tandem mass spectrometry techniques for the analysis of phosphopeptides
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Paris, Johanna, Theisen, Alina, Marzullo, Bryan P., Haris, Anisha, Morgan, Tomos E., Barrow, Mark P., O’Hara, John and O’Connor, Peter B. (2022) Multimodal tandem mass spectrometry techniques for the analysis of phosphopeptides. Journal of The American Society for Mass Spectrometry, 33 (7). pp. 1126-1133. doi:10.1021/jasms.1c00353 ISSN 1044-0305.
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Official URL: https://doi.org/10.1021/jasms.1c00353
Abstract
Collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), ultraviolet photodissociation (UVPD), electron capture dissociation and electron detachment dissociation (EDD) experiments were conducted on a set of phosphopeptides, in a Fourier transform ion cyclotron resonance mass spectrometer. The fragmentation patterns were compared and varied according to the fragmentation mechanisms and the composition of the peptides. CAD and IRMPD produced similar fragmentation profiles of the phosphopeptides, while UVPD produced a large number of complementary fragments. Electron-based dissociation techniques displayed lower fragmentation efficiencies, despite retaining the labile phosphate group, and drastically different fragmentation profiles. EDD produced complex spectra whose interpretation proved challenging.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QD Chemistry | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Tandem mass spectrometry, Phosphorylation , Multiphoton processes | ||||||||||||||||||
Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||||||||||||||||
Publisher: | Springer New York LLC | ||||||||||||||||||
ISSN: | 1044-0305 | ||||||||||||||||||
Official Date: | 6 July 2022 | ||||||||||||||||||
Dates: |
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Volume: | 33 | ||||||||||||||||||
Number: | 7 | ||||||||||||||||||
Page Range: | pp. 1126-1133 | ||||||||||||||||||
DOI: | 10.1021/jasms.1c00353 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 6 September 2022 | ||||||||||||||||||
Date of first compliant Open Access: | 7 September 2022 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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