Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

The in vitro effects of resistin on the innate immune signaling pathway in isolated human subcutaneous adipocytes

Tools
- Tools
+ Tools

Kusminski, C. M., Fernandez da Silva, Nancy, Creely, Steven J., Fisher, Ffolliott M., Harte, A. L. (Alison L.), Baker, Adam R., Kumar, Sudhesh and McTernan, P. G. (Philip G.) (2007) The in vitro effects of resistin on the innate immune signaling pathway in isolated human subcutaneous adipocytes. Journal of Clinical Endocrinology & Metabolism , Vol.92 (No.1). pp. 270-276. doi:10.1210/jc.2006-1151

[img]
Preview
PDF
WRAP_Kumar_2007_-_Post-print_The_in_vitro_effects_of_resistin.pdf - Requires a PDF viewer.

Download (208Kb)
Official URL: http://dx.doi.org/10.1210/jc.2006-1151

Request Changes to record.

Abstract

Context: Obesity-associated inflammation is a contributory factor in the pathogenesis of type 2 diabetes mellitus (T2DM); the mechanisms underlying the progression to T2DM are unclear. The adipokine resistin has demonstrated pro-inflammatory properties in relation to obesity and T2DM.
Objective: To characterize resistin expression in human obesity and address the role of resistin in the innate immune pathway. Furthermore, examine the influence of lipopolysaccharide, recombinant human resistin (rhResistin), insulin and rosiglitazone in human adipocytes. Finally, analyze the effect of rhResistin on the expression of components of the NF-κB pathway and insulin signaling cascade.
Methods: Abdominal subcutaneous adipose tissue was obtained from patients undergoing elective liposuction surgery (n = 35, aged: 36-49 yr; BMI: 26.5 ± 5.9 kg/m2). Isolated adipocytes were cultured with rhResistin (10-50 ng/ml). The level of cytokine secretion from isolated adipocytes was examined by ELISA. The effect of rhResistin on protein expression of components of the innate immune pathway was examined by Western blot.
Results: In-vitro studies demonstrated that antigenic stimuli increase resistin secretion (P < 0.001) from isolated adipocytes. Pro-inflammatory cytokine levels were increased in response to rhResistin (P < 0.001); this was attenuated by rosiglitazone (P < 0.01). When examining components of the innate immune pathway, rhResistin stimulated Toll-like receptor-2 protein expression. Similarly, mediators of the insulin signaling pathway, phosphospecific JNK1 and JNK2, were upregulated in response to rhResistin.
Conclusion: Resistin may participate in more than one mechanism to influence pro-inflammatory cytokine release from human adipocytes; potentially via the integration of NF-κB and JNK signaling pathways.

Item Type: Journal Article
Subjects: R Medicine > RC Internal medicine
Library of Congress Subject Headings (LCSH): Adipose tissues, Diabetes -- Research, Obesity
Journal or Publication Title: Journal of Clinical Endocrinology & Metabolism
Publisher: Endocrine Society
ISSN: 0021-972x
Official Date: 2007
Dates:
DateEvent
2007Published
Volume: Vol.92
Number: No.1
Page Range: pp. 270-276
DOI: 10.1210/jc.2006-1151
Status: Peer Reviewed
Access rights to Published version: Open Access
Description:

Version accepted by publisher (post-print, after peer review, before copy-editing).

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us