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Geometric principles underlying the proliferation of a model cell system
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Wu, Ling Juan, Lee, Seoungjun, Park, Sungshic, Eland, Lucy E., Wipat, Anil, Holden, Séamus and Errington, Jeff (2020) Geometric principles underlying the proliferation of a model cell system. Nature Communications, 11 (1). 4149 . doi:10.1038/s41467-020-17988-7 ISSN 2041-1723.
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Official URL: http://dx.doi.org/10.1038/s41467-020-17988-7
Abstract
Many bacteria can form wall-deficient variants, or L-forms, that divide by a simple mechanism that does not require the FtsZ-based cell division machinery. Here, we use microfluidic systems to probe the growth, chromosome cycle and division mechanism of Bacillus subtilis L-forms. We find that forcing cells into a narrow linear configuration greatly improves the efficiency of cell growth and chromosome segregation. This reinforces the view that L-form division is driven by an excess accumulation of surface area over volume. Cell geometry also plays a dominant role in controlling the relative positions and movement of segregating chromosomes. Furthermore, the presence of the nucleoid appears to influence division both via a cell volume effect and by nucleoid occlusion, even in the absence of FtsZ. Our results emphasise the importance of geometric effects for a range of crucial cell functions, and are of relevance for efforts to develop artificial or minimal cell systems.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Nature Communications | ||||||
Publisher: | Nature Publishing Group | ||||||
ISSN: | 2041-1723 | ||||||
Official Date: | 18 August 2020 | ||||||
Dates: |
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Volume: | 11 | ||||||
Number: | 1 | ||||||
Article Number: | 4149 | ||||||
DOI: | 10.1038/s41467-020-17988-7 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
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