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High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization
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Holden, Séamus, Pengo, Thomas, Meibom, Karin L., Fernandez Fernandez, Carmen, Collier, Justine and Manley, Suliana (2014) High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization. Proceedings of the National Academy of Sciences of the United States of America, 111 (12). pp. 4566-4571. doi:10.1073/pnas.1313368111 ISSN 0027-8424.
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Official URL: http://dx.doi.org/10.1073/pnas.1313368111
Abstract
We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of “Z-ring” organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||||
Publisher: | National Academy of Sciences | ||||||
ISSN: | 0027-8424 | ||||||
Official Date: | 25 March 2014 | ||||||
Dates: |
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Volume: | 111 | ||||||
Number: | 12 | ||||||
Page Range: | pp. 4566-4571 | ||||||
DOI: | 10.1073/pnas.1313368111 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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