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Photoactivated localization microscopy for cellular imaging
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Achurra, Paulina, Holden, Séamus, Pengo, Thomas and Manley, Suliana (2014) Photoactivated localization microscopy for cellular imaging. In: Super-Resolution Microscopy Techniques in the Neurosciences. Neuromethods, 86 . Totowa, NJ: Humana Press, pp. 87-111. ISBN 9781627039826
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Official URL: http://dx.doi.org/10.1007/978-1-62703-983-3_5
Abstract
In a method termed photoactivated localization microscopy (PALM), super-resolution fluorescence imaging can be achieved through the localization of single molecules. This allows the resolution of specific proteins fused to the appropriate fluorescent protein label. Here, we summarize fluorescent proteins suitable for PALM, the technical aspects of multicolor and three-dimensional imaging, and the software packages that are available. Additionally, we highlight several biological applications with an emphasis on neuroscience.
| Item Type: | Book Item | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Series Name: | Neuromethods | ||||
| Publisher: | Humana Press | ||||
| Place of Publication: | Totowa, NJ | ||||
| ISBN: | 9781627039826 | ||||
| ISSN: | 0893-2336 | ||||
| Book Title: | Super-Resolution Microscopy Techniques in the Neurosciences | ||||
| Official Date: | 21 January 2014 | ||||
| Dates: |
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| Volume: | 86 | ||||
| Page Range: | pp. 87-111 | ||||
| DOI: | 10.1007/978-1-62703-983-3_5 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published |
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