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Single-molecule DNA biosensors for quantitative transcription factor detection
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Crawford, Robert, Lymperopoulos, Konstantinos, Torella, Joseph P., Heilemann, Mike, Hwang, Ling C., Holden, Séamus and Kapanidis, Achillefs (2010) Single-molecule DNA biosensors for quantitative transcription factor detection. Biophysical Journal, 98 (3). 611a. doi:10.1016/j.bpj.2009.12.3333 ISSN 0006-3495.
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Official URL: http://dx.doi.org/10.1016/j.bpj.2009.12.3333
Abstract
We have developed two single-molecule fluorescence biosensors that can quantitatively detect single or multiple transcription factors (TFs) with high sensitivity and specificity. The first sensor is based on TF-based coincidence of two DNA fragments, each containing half of a particular TF binding sequence. In the absence of the particular transcription factor, the DNA fragments diffuse independently. The coincidence is detectable using 2-colour alternating laser excitation (ALEX) spectroscopy using either solution or surface-based approaches.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Biophysical Journal | ||||
Publisher: | Biophysical Society | ||||
ISSN: | 0006-3495 | ||||
Official Date: | 1 January 2010 | ||||
Dates: |
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Volume: | 98 | ||||
Number: | 3 | ||||
Article Number: | 611a | ||||
DOI: | 10.1016/j.bpj.2009.12.3333 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Copyright Holders: | © 2010 Biophysical Society. Published by Elsevier Inc. |
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