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Asgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery
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Hatano, Tomoyuki, Palani, Saravanan, Papatziamou, Dimitra, Salzer, Ralf, Souza, Diorge P., Tamarit, Daniel, Makwana, Mehul, Potter, Antonia, Haig, Alexandra, Xu, Wenjue, Townsend, David, Rochester, David, Bellini, Dom, Hussain, Hamdi M. A., Ettema, Thijs J. G., Löwe, Jan, Baum, Buzz, Robinson, Nicholas P. and Balasubramanian, Mohan K. (2022) Asgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery. Nature Communications, 13 (1). 3398. doi:10.1038/s41467-022-30656-2 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-022-30656-2
Abstract
The ESCRT machinery, comprising of multiple proteins and subcomplexes, is crucial for membrane remodelling in eukaryotic cells, in processes that include ubiquitin-mediated multivesicular body formation, membrane repair, cytokinetic abscission, and virus exit from host cells. This ESCRT system appears to have simpler, ancient origins, since many archaeal species possess homologues of ESCRT-III and Vps4, the components that execute the final membrane scission reaction, where they have been shown to play roles in cytokinesis, extracellular vesicle formation and viral egress. Remarkably, metagenome assemblies of Asgard archaea, the closest known living relatives of eukaryotes, were recently shown to encode homologues of the entire cascade involved in ubiquitin-mediated membrane remodelling, including ubiquitin itself, components of the ESCRT-I and ESCRT-II subcomplexes, and ESCRT-III and Vps4. Here, we explore the phylogeny, structure, and biochemistry of Asgard homologues of the ESCRT machinery and the associated ubiquitylation system. We provide evidence for the ESCRT-I and ESCRT-II subcomplexes being involved in ubiquitin-directed recruitment of ESCRT-III, as it is in eukaryotes. Taken together, our analyses suggest a pre-eukaryotic origin for the ubiquitin-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.
Item Type: | Journal Article | |||||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Eukaryotic cells , Cell membranes, Biochemistry, Cell physiology, Ubiquitin | |||||||||||||||||||||||||||
Journal or Publication Title: | Nature Communications | |||||||||||||||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||||||||||||||
ISSN: | 2041-1723 | |||||||||||||||||||||||||||
Official Date: | 13 June 2022 | |||||||||||||||||||||||||||
Dates: |
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Volume: | 13 | |||||||||||||||||||||||||||
Number: | 1 | |||||||||||||||||||||||||||
Article Number: | 3398 | |||||||||||||||||||||||||||
DOI: | 10.1038/s41467-022-30656-2 | |||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
Date of first compliant deposit: | 22 November 2022 | |||||||||||||||||||||||||||
Date of first compliant Open Access: | 23 November 2022 | |||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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