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Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori

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Kulick, Stefan, Moccia, Claudia, Didelot, Xavier, Falush, Daniel, Kraft, Christian and Suerbaum, Sebastian. (2008) Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PLOS One, Vol.3 (No.11). ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0003797

Abstract

Helicobacter pylori colonizes the gastric mucosa of half of the human population, causing gastritis, ulcers, and cancer. H. pylori is naturally competent for transformation by exogenous DNA, and recombination during mixed infections of one stomach with multiple H. pylori strains generates extensive allelic diversity. We developed an in vitro transformation protocol to study genomic imports after natural transformation of H. pylori. The mean length of imported fragments was dependent on the combination of donor and recipient strain and varied between 1294 bp and 3853 bp. In about 10% of recombinant clones, the imported fragments of donor DNA were interrupted by short interspersed sequences of the recipient (ISR) with a mean length of 82 bp. 18 candidate genes were inactivated in order to identify genes involved in the control of import length and generation of ISR. Inactivation of the antimutator glycosylase MutY increased the length of imports, but did not have a significant effect on ISR frequency. Overexpression of mutY strongly increased the frequency of ISR, indicating that MutY, while not indispensable for ISR formation, is part of at least one ISR-generating pathway. The formation of ISR in H. pylori increases allelic diversity, and contributes to the uniquely low linkage disequilibrium characteristic of this pathogen.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Statistics
Library of Congress Subject Headings (LCSH):	Helicobacter pylori -- Genetics, Genetic recombination
Journal or Publication Title:	PLOS One
Publisher:	Public Library of Science
ISSN:	1932-6203
Date:	24 November 2008
Volume:	Vol.3
Number:	No.11
Number of Pages:	9
Identification Number:	10.1371/journal.pone.0003797
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Sixth Framework Programme (European Commission) (FP6), Deutsche Forschungsgemeinschaft (DFG), Deutscher Akademischer Austauschdienst (DAAD), Wilhelm Hirte Foundation
Grant number:	LSHC-CT-2005-018704 (FP6), GRK745 (DFG)
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URI:	http://wrap.warwick.ac.uk/id/eprint/16660