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Structure of cytosine transport protein CodB provides insight into nucleobase‐cation symporter 1 mechanism

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Hatton, Caitlin, Brotherton, Deborah H., Spencer, Mahalah and Cameron, Alexander D. (2022) Structure of cytosine transport protein CodB provides insight into nucleobase‐cation symporter 1 mechanism. The EMBO Journal, 41 (16). e110527. doi:10.15252/embj.2021110527 ISSN 0261-4189.

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Official URL: https://doi.org/10.15252/embj.2021110527

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Abstract

CodB is a cytosine transporter from the Nucleobase‐Cation‐Symport‐1 (NCS1) transporter family, a member of the widespread LeuT superfamily. Previous experiments with the nosocomial pathogen Pseudomonas aeruginosa have shown CodB as also important for the uptake of 5‐fluorocytosine, which has been suggested as a novel drug to combat antimicrobial resistance by suppressing virulence. Here we solve the crystal structure of CodB from Proteus vulgaris, at 2.4 Å resolution in complex with cytosine. We show that CodB carries out the sodium‐dependent uptake of cytosine and can bind 5‐fluorocytosine. Comparison of the substrate‐bound structures of CodB and the hydantoin transporter Mhp1, the only other NCS1 family member for which the structure is known, highlight the importance of the hydrogen bonds that the substrates make with the main chain at the breakpoint in the discontinuous helix, TM6. In contrast to other LeuT superfamily members, neither CodB nor Mhp1 makes specific interactions with residues on TM1. Comparison of the structures provides insight into the intricate mechanisms of how these proteins transport substrates across the plasma membrane.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Q Science > QR Microbiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Cytosine , Carrier proteins, Membrane proteins -- Physiological transport, Drug resistance in microorganisms -- Effect of drugs on
Journal or Publication Title: The EMBO Journal
Publisher: EMBO Press
ISSN: 0261-4189
Official Date: 16 August 2022
Dates:
DateEvent
16 August 2022Published
1 July 2022Available
26 May 2022Accepted
Volume: 41
Number: 16
Article Number: e110527
DOI: 10.15252/embj.2021110527
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 18 July 2022
Date of first compliant Open Access: 19 July 2022
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
1782611[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
MR/P010393/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265

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