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Chemical inhibition of auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis
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Fukui, Kosuke, Arai, Kazushi, Tanaka, Yuka, Aoi, Yuki, Kukshal, Vandna, Jez, Joseph M., Kubeš, Martin F., Napier, Richard, Zhao, Yunde, Kasahara, Hiroyuki and Hayashi, Ken-ichiro (2022) Chemical inhibition of auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis. Proceedings of the National Academy of Sciences, 119 (32). e2206869119. doi:10.1073/pnas.2206869119 ISSN 0027-8424.
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Official URL: https://doi.org/10.1073/pnas.2206869119
Abstract
The phytohormone auxin, indole-3-acetic acid (IAA), plays a prominent role in plant development. Auxin homeostasis is coordinately regulated by auxin synthesis, transport, and inactivation; however, the physiological contribution of auxin inactivation to auxin homeostasis has not been determined. The GH3 IAA–amino acid conjugating enzymes play a central role in auxin inactivation. Chemical inhibition of GH3 proteins in planta is challenging because the inhibition of these enzymes leads to IAA overaccumulation that rapidly induces GH3 expression. Here, we report the characterization of a potent GH3 inhibitor, kakeimide, that selectively targets IAA-conjugating GH3 proteins. Chemical knockdown of the auxin inactivation pathway demonstrates that auxin turnover is very rapid (about 10 min) and indicates that both auxin biosynthesis and inactivation dynamically regulate auxin homeostasis.
Item Type: | Journal Article | ||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QK Botany Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Auxin , Plants -- Effect of auxin on , Plant hormones , Chemical biology , Homeostasis | ||||||||||||||||||||||||
Journal or Publication Title: | Proceedings of the National Academy of Sciences | ||||||||||||||||||||||||
Publisher: | National Academy of Sciences | ||||||||||||||||||||||||
ISSN: | 0027-8424 | ||||||||||||||||||||||||
Official Date: | 1 August 2022 | ||||||||||||||||||||||||
Dates: |
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Volume: | 119 | ||||||||||||||||||||||||
Number: | 32 | ||||||||||||||||||||||||
Article Number: | e2206869119 | ||||||||||||||||||||||||
DOI: | 10.1073/pnas.2206869119 | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
Date of first compliant deposit: | 4 July 2022 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 5 August 2022 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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