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Impact of environmentally relevant concentrations of Bisphenol A (BPA) on the gene expression profile in an in vitro model of the normal human ovary
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Zahra, Aeman, Kerslake, Rachel, Kyrou, Ioannis, Randeva, Harpal S., Sisu, Cristina and Karteris, Emmanouil (2022) Impact of environmentally relevant concentrations of Bisphenol A (BPA) on the gene expression profile in an in vitro model of the normal human ovary. International Journal of Molecular Sciences, 23 (10). 5334. doi:10.3390/ijms23105334 ISSN 1422-0067.
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WRAP-Impact-of-environmentally-relevant-concentrations-of-Bisphenol-A-BPA-on-the-gene-expression-profile-Kyrou-22.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3660Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/ijms23105334
Abstract
Endocrine-disrupting chemicals (EDCs), including the xenoestrogen Bisphenol A (BPA), can interfere with hormonal signalling. Despite increasing reports of adverse health effects associated with exposure to EDCs, there are limited data on the effect of BPA in normal human ovaries. In this paper, we present a detailed analysis of the transcriptomic landscape in normal Human Epithelial Ovarian Cells (HOSEpiC) treated with BPA (10 and 100 nM). Gene expression profiles were determined using high-throughput RNA sequencing, followed by functional analyses using bioinformatics tools. In total, 272 and 454 differentially expressed genes (DEGs) were identified in 10 and 100 nM BPA-treated HOSEpiCs, respectively, compared to untreated controls. Biological pathways included mRNA surveillance pathways, oocyte meiosis, cellular senescence, and transcriptional misregulation in cancer. BPA exposure has a considerable impact on 10 genes: ANAPC2, AURKA, CDK1, CCNA2, CCNB1, PLK1, BUB1, KIF22, PDE3B, and CCNB3, which are also associated with progesterone-mediated oocyte maturation pathways. Future studies should further explore the effects of BPA and its metabolites in the ovaries in health and disease, making use of validated in vitro and in vivo models to generate data that will address existing knowledge gaps in basic biology, hazard characterisation, and risk assessment associated with the use of xenoestrogens such as BPA.
| Item Type: | Journal Article | ||||||||
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| Subjects: | R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||
| Library of Congress Subject Headings (LCSH): | Endocrine disrupting chemicals, Endocrine toxicology, Endocrine glands -- Effect of chemicals on, Bisphenol A -- Toxicology, Bisphenol A -- Environmental aspects, Ovaries -- Cancer | ||||||||
| Journal or Publication Title: | International Journal of Molecular Sciences | ||||||||
| Publisher: | M D P I AG | ||||||||
| ISSN: | 1422-0067 | ||||||||
| Official Date: | 10 May 2022 | ||||||||
| Dates: |
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| Volume: | 23 | ||||||||
| Number: | 10 | ||||||||
| Number of Pages: | 14 | ||||||||
| Article Number: | 5334 | ||||||||
| DOI: | 10.3390/ijms23105334 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 4 July 2022 | ||||||||
| Date of first compliant Open Access: | 5 July 2022 | ||||||||
| RIOXX Funder/Project Grant: |
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