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Adipocyte-breast cancer cell co-culture in Tswells
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Ayaani, Soofiyah Parveen, Wabitsch, Martin and Christian, Mark (2022) Adipocyte-breast cancer cell co-culture in Tswells. In: Christian, S. L., (ed.) Cancer Cell Biology. Methods in Molecular Biology, 2508 . Springer, pp. 59-68. ISBN 9781071623756
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Official URL: https://doi.org/10.1007/978-1-0716-2376-3_6
Abstract
Experiments with cell co-culture systems facilitate investigation of the effects of one cell population on another, when the cells are grown in close proximity. Here we describe co-culture of Simpson-Golabi-Behmel syndrome (SGBS) adipocyte cells with the MCF-7 breast cancer cell line using the Corning Transwell 12-mm, 0.4-μm pore polyester membrane insert cell culture system. The SGBS adipocyte cell line, which was developed from cells taken from an infant with Simpson-Golabi-Behmel syndrome is comparable, both functionally and biochemically, to primary preadipocytes. The MCF-7 breast cancer cell line is an ER /PR and HER2 line used very commonly in studies of breast malignancy. Consisting of insert supports with a permeable membrane 'floor,' which sit suspended in wells, the Corning Transwell co-culture system allows communication between physically separate cells cultured on the membrane and in the well beneath. This co-culture procedure described here can be applied to analyze the effects of cancer cells on the process of adipogenesis and the changes in cancer cells due to adipocyte-secreted factors.
| Item Type: | Book Item | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
| SWORD Depositor: | Library Publications Router | ||||
| Series Name: | Methods in Molecular Biology | ||||
| Publisher: | Springer | ||||
| ISBN: | 9781071623756 | ||||
| ISSN: | 1064-3745 | ||||
| Book Title: | Cancer Cell Biology | ||||
| Editor: | Christian, S. L. | ||||
| Official Date: | 24 June 2022 | ||||
| Dates: |
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| Volume: | 2508 | ||||
| Page Range: | pp. 59-68 | ||||
| DOI: | 10.1007/978-1-0716-2376-3_6 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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