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Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development
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Mau, King Hang Tommy, Karimlou, Donja, Barneda, David, Brochard, Vincent, Royer, Christophe, Leeke, Bryony, de Souza, Roshni A., Pailles, Mélanie, Percharde, Michelle, Srinivas, Shankar, Jouneau, Alice, Christian, Mark and Azuara, Véronique (2022) Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development. Nature Communications, 13 (1). 3861. doi:10.1038/s41467-022-31323-2 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-022-31323-2
Abstract
Mammalian pre-implantation embryos accumulate substantial lipids, which are stored in lipid droplets (LDs). Despite the fundamental roles of lipids in many cellular functions, the significance of building-up LDs for the developing embryo remains unclear. Here we report that the accumulation and mobilization of LDs upon implantation are causal in the morphogenesis of the pluripotent epiblast and generation of the pro-amniotic cavity in mouse embryos, a critical step for all subsequent development. We show that the CIDEA protein, found abundantly in adipocytes, enhances lipid storage in blastocysts and pluripotent stem cells by promoting LD enlargement through fusion. The LD-stored lipids are mobilized into lysosomes at the onset of lumenogenesis, but without CIDEA are prematurely degraded by cytosolic lipases. Loss of lipid storage or inactivation of lipophagy leads to the aberrant formation of multiple cavities within disorganised epithelial structures. Thus, our study reveals an unexpected role for LDs in orchestrating tissue remodelling and uncovers underappreciated facets of lipid metabolism in peri-implantation development.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QL Zoology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Lipids , Membrane lipids, Morphogenesis , Multipotent stem cells, Cell culture, Stem cells | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Nature Communications | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Publisher: | Springer Science and Business Media LLC | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ISSN: | 2041-1723 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Official Date: | 5 July 2022 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Volume: | 13 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Number: | 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Article Number: | 3861 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1038/s41467-022-31323-2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 26 July 2022 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 26 July 2022 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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