The Library
Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants
Tools
Tools
Tools
Murray, Kathryn, Kinney, Nina L. H., Griffiths, Christopher A., Hasan, Muhammad, Gibson, Matthew I. and Whale, Thomas F. (2022) Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants. Scientific Reports, 12 (1). 12295. doi:10.1038/s41598-022-15545-4 ISSN 2045-2322.
|
PDF
WRAP-pollen-derived-macromolecules-serve-new-class-ice-nucleating-cryoprotectants-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1769Kb) | Preview |
Official URL: https://doi.org/10.1038/s41598-022-15545-4
Abstract
Cryopreservation of biological material is vital for existing and emerging biomedical and biotechnological research and related applications, but there remain significant challenges. Cryopreservation of cells in sub-milliliter volumes is difficult because they tend to deeply supercool, favoring lethal intracellular ice formation. Some tree pollens are known to produce polysaccharides capable of nucleating ice at warm sub-zero temperatures. Here we demonstrated that aqueous extractions from European hornbeam pollen (pollen washing water, PWW) increased ice nucleation temperatures in 96-well plates from ≈ − 13 °C to ≈ − 7 °C. Application of PWW to the cryopreservation of immortalized T-cells in 96-well plates resulted in an increase of post-thaw metabolic activity from 63.9% (95% CI [58.5 to 69.2%]) to 97.4% (95% CI [86.5 to 108.2%]) of unfrozen control. When applied to cryopreservation of immortalized lung carcinoma monolayers, PWW dramatically increased post-thaw metabolic activity, from 1.6% (95% CI [− 6.6 to 9.79%]) to 55.0% (95% CI [41.6 to 68.4%]). In contrast to other ice nucleating agents, PWW is soluble, sterile and has low cytotoxicity meaning it can be readily incorporated into existing cryopreservation procedures. As such, it can be regarded as a unique class of cryoprotectant which acts by inducing ice nucleation at warm temperatures.
| Item Type: | Journal Article | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QK Botany |
|||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cryobiology, Cryopreservation of organs, tissues, etc., Pollen, Ice nuclei, Cold adaptation | |||||||||||||||||||||||||||
| Journal or Publication Title: | Scientific Reports | |||||||||||||||||||||||||||
| Publisher: | Nature Publishing Group | |||||||||||||||||||||||||||
| ISSN: | 2045-2322 | |||||||||||||||||||||||||||
| Official Date: | 19 July 2022 | |||||||||||||||||||||||||||
| Dates: |
|
|||||||||||||||||||||||||||
| Volume: | 12 | |||||||||||||||||||||||||||
| Number: | 1 | |||||||||||||||||||||||||||
| Article Number: | 12295 | |||||||||||||||||||||||||||
| DOI: | 10.1038/s41598-022-15545-4 | |||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
| Date of first compliant deposit: | 20 July 2022 | |||||||||||||||||||||||||||
| Date of first compliant Open Access: | 22 July 2022 | |||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
