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Phage engineering and phage-assisted CRISPR-Cas delivery to combat multidrug-resistant pathogens
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Khambhati, Khushal, Bhattacherjee, Gargi, Gohil, Nisarg, Dhanoa, Gurneet, Sagona, Antonia P., Mani, Indra, Bui, Nhat Le, Chu, Dinh-Toi, Karapurkar, Janardhan, Jang, Su Hwa, Chung, Hee Yong, Maurya, Rupesh, Alzahrani, Khalid, Ramakrishna, Suresh and Singh, Vijai (2023) Phage engineering and phage-assisted CRISPR-Cas delivery to combat multidrug-resistant pathogens. Bioengineering & Translational Medicine, 8 (2). e10381. doi:10.1002/btm2.10381 ISSN 2380-6761.
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WRAP-Phage-engineering-phage-assisted-CRISPR-Cas-delivery-combat-multidrug-resistant-pathogens-Sagona-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3151Kb) | Preview |
Official URL: https://doi.org/10.1002/btm2.10381
Abstract
Antibiotic resistance ranks among the top threats to humanity. Due to the frequent use of antibiotics, society is facing a high prevalence of multidrug resistant pathogens, which have managed to evolve mechanisms that help them evade the last line of therapeutics. An alternative to antibiotics could involve the use of bacteriophages (phages), which are the natural predators of bacterial cells. In earlier times, phages were implemented as therapeutic agents for a century but were mainly replaced with antibiotics, and considering the menace of antimicrobial resistance, it might again become of interest due to the increasing threat of antibiotic resistance among pathogens. The current understanding of phage biology and clustered regularly interspaced short palindromic repeats (CRISPR) assisted phage genome engineering techniques have facilitated to generate phage variants with unique therapeutic values. In this review, we briefly explain strategies to engineer bacteriophages. Next, we highlight the literature supporting CRISPR-Cas9-assisted phage engineering for effective and more specific targeting of bacterial pathogens. Lastly, we discuss techniques that either help to increase the fitness, specificity, or lytic ability of bacteriophages to control an infection.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | Bioengineering & Translational Medicine | ||||||||
Publisher: | John Wiley & Sons, Inc. | ||||||||
ISSN: | 2380-6761 | ||||||||
Official Date: | March 2023 | ||||||||
Dates: |
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Volume: | 8 | ||||||||
Number: | 2 | ||||||||
Number of Pages: | 23 | ||||||||
Article Number: | e10381 | ||||||||
DOI: | 10.1002/btm2.10381 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 19 July 2022 | ||||||||
Date of first compliant Open Access: | 8 August 2022 | ||||||||
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