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Copper abolishes the β-sheet secondary structure of preformed amyloid fibrils of amyloid-β42
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House, Emily, Mold, Matthew, Collingwood, Joanna F., Baldwin, Alex, Goodwin, Steven and Exley, Christopher. (2009) Copper abolishes the β-sheet secondary structure of preformed amyloid fibrils of amyloid-β42. Journal of Alzheimer's disease : JAD, Vo.18 (No.4). pp. 811-817. ISSN 1387-2877
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Official URL: http://dx.doi.org/10.3233/JAD-2009-1235
Abstract
The observation of the co-deposition of metals and amyloid-beta(42) (A beta(42)) in brain tissue in Alzheimer's disease prompted myriad investigations into the role played by metals in the precipitation of this peptide. Copper is bound by monomeric A beta(42) and upon precipitation of the copper-peptide complex thereby prevents A beta(42) from adopting a beta-sheet secondary structure. Copper is also bound by beta-sheet conformers of A beta(42), and herein we have investigated how this interaction affects the conformation of the precipitated peptide. Copper significantly reduced the thioflavin T fluorescence of aged, fibrillar A beta(42) with, for example, a 20-fold excess of the metal resulting in a ca 90% reduction in thioflavin T fluorescence. Transmission electron microscopy showed that copper significantly reduced the quantities of amyloid fibrils while Congo red staining and polarized light demonstrated a copper-induced abolition of apple-green birefringence. Microscopy under cross-polarized light also revealed the first observation of spherulites of A beta(42). The size and appearance of these amyloid structures were found to be very similar to spherulites identified in Alzheimer's disease tissue. The combined results of these complementary methods strongly suggested that copper abolished the beta-sheet secondary structure of pre-formed, aged amyloid fibrils of A beta(42). Copper may protect against the presence of beta-sheets of A beta(42) in vivo, and its binding by fibrillar A beta(42) could have implications for Alzheimer's disease therapy.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry R Medicine > RC Internal medicine T Technology > TP Chemical technology |
| Divisions: | Faculty of Science > Engineering |
| Library of Congress Subject Headings (LCSH): | Alzheimer's disease, Aluminum, Amyloid, Congo red, Copper, Precipitation (Chemistry) |
| Journal or Publication Title: | Journal of Alzheimer's disease : JAD |
| Publisher: | IOS Press |
| ISSN: | 1387-2877 |
| Date: | August 2009 |
| Volume: | Vo.18 |
| Number: | No.4 |
| Number of Pages: | 7 |
| Page Range: | pp. 811-817 |
| Identification Number: | 10.3233/JAD-2009-1235 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Engineering and Physical Sciences Research Council (EPSRC), National Institutes of Health (U.S.) (NIH) |
| Grant number: | EP/D066654/1 (EPSRC), R21NS060304 (NIH) |
| URI: | http://wrap.warwick.ac.uk/id/eprint/16772 |
Data sourced from Thomson Reuters' Web of Knowledge
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