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Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation
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Kuey, Cansu, Sittewelle, Méghane, Larocque, Gabrielle, Hernandez-González, Miguel and Royle, Stephen J. (2022) Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. eLife, 11 . e78929. doi:10.7554/elife.78929
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WRAP-recruitment-clathrin-intracellular-membranes-sufficient-vesicle formation-Royle-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (9Mb) | Preview |
Official URL: https://doi.org/10.7554/elife.78929
Abstract
The formation of a clathrin-coated vesicle (CCV) is a major membrane remodeling process that is crucial for membrane traffic in cells. Besides clathrin, these vesicles contain at least 100 different proteins although it is unclear how many are essential for the formation of the vesicle. Here, we show that intracellular clathrin-coated formation can be induced in living cells using minimal machinery and that it can be achieved on various membranes, including the mitochondrial outer membrane. Chemical heterodimerization was used to inducibly attach a clathrin-binding fragment ‘hook’ to an ‘anchor’ protein targeted to a specific membrane. Endogenous clathrin assembled to form coated pits on the mitochondria, termed MitoPits, within seconds of induction. MitoPits are double-membraned invaginations that form preferentially on high curvature regions of the mitochondrion. Upon induction, all stages of CCV formation – initiation, invagination, and even fission – were faithfully reconstituted. We found no evidence for the functional involvement of accessory proteins in this process. In addition, fission of MitoPit-derived vesicles was independent of known scission factors including dynamins and dynamin-related protein 1 (Drp1), suggesting that the clathrin cage generates sufficient force to bud intracellular vesicles. Our results suggest that, following its recruitment, clathrin is sufficient for intracellular CCV formation.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics | |||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cell membranes -- Research, Cytology -- Research, Endocytosis -- Research, Proteins -- Physiological transport, Biological transport | |||||||||||||||
| Journal or Publication Title: | eLife | |||||||||||||||
| Publisher: | eLife Sciences Publications Ltd. | |||||||||||||||
| ISSN: | 2050-084X | |||||||||||||||
| Official Date: | 19 July 2022 | |||||||||||||||
| Dates: |
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| Volume: | 11 | |||||||||||||||
| Article Number: | e78929 | |||||||||||||||
| DOI: | 10.7554/elife.78929 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||
| RIOXX Funder/Project Grant: |
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