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Capturing the mechanics of clathrin-mediated endocytosis
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Smith, Sarah M. and Smith, Corinne J. (2022) Capturing the mechanics of clathrin-mediated endocytosis. Current Opinion in Structural Biology, 75 . 102427. doi:10.1016/j.sbi.2022.102427 ISSN 0959-440X.
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WRAP-Capturing-the-mechanics-of-clathrin-mediated-endocytosis-Smith-22.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2619Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.sbi.2022.102427
Abstract
Clathrin-mediated endocytosis enables selective uptake of molecules into cells in response to changing cellular needs. It occurs through assembly of coat components around the plasma membrane that determine vesicle contents and facilitate membrane bending to form a clathrin-coated transport vesicle. In this review we discuss recent cryo-electron microscopy structures that have captured a series of events in the life cycle of a clathrin-coated vesicle. Both single particle analysis and tomography approaches have revealed details of the clathrin lattice structure itself, how AP2 may interface with clathrin within a coated vesicle and the importance of PIP2 binding for assembly of the yeast adaptors Sla2 and Ent1 on the membrane. Within cells, cryo-electron tomography of clathrin in flat lattices and high-speed AFM studies provided new insights into how clathrin morphology can adapt during CCV formation. Thus, key mechanical processes driving clathrin-mediated endocytosis have been captured through multiple techniques working in partnership.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Cell membranes , Endocytosis | |||||||||||||||
Journal or Publication Title: | Current Opinion in Structural Biology | |||||||||||||||
Publisher: | Elsevier | |||||||||||||||
ISSN: | 0959-440X | |||||||||||||||
Official Date: | August 2022 | |||||||||||||||
Dates: |
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Volume: | 75 | |||||||||||||||
Number of Pages: | 9 | |||||||||||||||
Article Number: | 102427 | |||||||||||||||
DOI: | 10.1016/j.sbi.2022.102427 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 2 August 2022 | |||||||||||||||
Date of first compliant Open Access: | 3 August 2022 | |||||||||||||||
RIOXX Funder/Project Grant: |
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