Vanoosthuyse, Vincent, Meadows, John C., van der Sar, Sjaak J. A., Millar, Jonathan B. A. and Hardwick, Kevin G. (2009) Bub3p facilitates spindle checkpoint silencing in fission yeast. Molecular Biology of the Cell, Vol.20 (No.24). pp. 5096-5105. doi:10.1091/mbc.E09-09-0762

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Abstract

Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochores in fission yeast cells lacking Bub3p. Surprisingly, a robust mitotic arrest is maintained in the majority of bub3 Delta cells, yet they die, suggesting that Bub3p is essential for successful checkpoint recovery. During recovery, two defects are observed: (1) cells mis-segregate chromosomes and (2) anaphase onset is significantly delayed. We show that Bub3p is required to activate the APC upon inhibition of Aurora kinase activity in checkpoint-arrested cells, suggesting that Bub3p is required for efficient checkpoint silencing downstream of Aurora kinase. Together, these results suggest that spindle checkpoint signals can be amplified in the nucleoplasm, yet kinetochore localization of spindle checkpoint components is required for proper recovery from a spindle checkpoint-dependent arrest.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Spindle (Cell division), Schizosaccharomyces pombe, Microtubules
Journal or Publication Title:	Molecular Biology of the Cell
Publisher:	Amer Soc Cell Biology
ISSN:	1059-1524
Official Date:	15 December 2009
Dates:	Date Event 15 December 2009 Published
Volume:	Vol.20
Number:	No.24
Number of Pages:	10
Page Range:	pp. 5096-5105
DOI:	10.1091/mbc.E09-09-0762
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Wellcome Trust (London, England), Medical Research Council (Great Britain) (MRC)

Data sourced from Thomson Reuters' Web of Knowledge

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