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Bub3p facilitates spindle checkpoint silencing in fission yeast
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Vanoosthuyse, Vincent, Meadows, John C., van der Sar, Sjaak J. A., Millar, Jonathan B. A. and Hardwick, Kevin G. (2009) Bub3p facilitates spindle checkpoint silencing in fission yeast. Molecular Biology of the Cell, Vol.20 (No.24). pp. 5096-5105. doi:10.1091/mbc.E09-09-0762 ISSN 1059-1524.
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Official URL: http://dx.doi.org/10.1091/mbc.E09-09-0762
Abstract
Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochores in fission yeast cells lacking Bub3p. Surprisingly, a robust mitotic arrest is maintained in the majority of bub3 Delta cells, yet they die, suggesting that Bub3p is essential for successful checkpoint recovery. During recovery, two defects are observed: (1) cells mis-segregate chromosomes and (2) anaphase onset is significantly delayed. We show that Bub3p is required to activate the APC upon inhibition of Aurora kinase activity in checkpoint-arrested cells, suggesting that Bub3p is required for efficient checkpoint silencing downstream of Aurora kinase. Together, these results suggest that spindle checkpoint signals can be amplified in the nucleoplasm, yet kinetochore localization of spindle checkpoint components is required for proper recovery from a spindle checkpoint-dependent arrest.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Spindle (Cell division), Schizosaccharomyces pombe, Microtubules | ||||
Journal or Publication Title: | Molecular Biology of the Cell | ||||
Publisher: | Amer Soc Cell Biology | ||||
ISSN: | 1059-1524 | ||||
Official Date: | 15 December 2009 | ||||
Dates: |
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Volume: | Vol.20 | ||||
Number: | No.24 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 5096-5105 | ||||
DOI: | 10.1091/mbc.E09-09-0762 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Wellcome Trust (London, England), Medical Research Council (Great Britain) (MRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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