The Library
The Arabidopsis WRR4A and WRR4B paralogous NLR proteins both confer recognition of multiple Albugo candida effectors
Tools
Redkar, Amey, Cevik, Volkan, Bailey, Kate, Zhao, He, Kim, Dae Sung, Zou, Zhou, Furzer, Oliver J., Fairhead, Sebastian, Borhan, M. Hossein, Holub, E. B. and Jones, Jonathan D. G. (2023) The Arabidopsis WRR4A and WRR4B paralogous NLR proteins both confer recognition of multiple Albugo candida effectors. New Phytologist, 237 (2). pp. 532-547. doi:10.1111/nph.18378 ISSN 0028-646X.
|
PDF
WRAP-Arabidopsis-WRR4A-WRR4B-paralogous-NLR-proteins-both-confer-recognition-multiple-Albugo-candida-effectors-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (5Mb) | Preview |
Official URL: https://doi.org/10.1111/nph.18378
Abstract
Summary: The oomycete Albugo candida causes white blister rust, an important disease of Brassica crops. Distinct races of A. candida are defined by their capacity to infect different host plant species. Each A. candida race encodes secreted proteins with a CX2CX5G (‘CCG’) motif that are polymorphic and show presence/absence variation, and are therefore candidate effectors. The White Rust Resistance 4 (WRR4) locus in Arabidopsis thaliana accession Col‐0 contains three genes that encode intracellular nucleotide‐binding domain leucine‐rich repeat immune receptors. The Col‐0 alleles of WRR4A and WRR4B confer resistance to multiple A. candida races, although both WRR4A and WRR4B can be overcome by the Col‐0‐virulent race 4 isolate AcEx1. Comparison of CCG candidate effectors in avirulent and virulent races, and transient co‐expression of CCG effectors from four A. candida races in Nicotiana sp. or A. thaliana, revealed CCG effectors that trigger WRR4A‐ or WRR4B‐dependent hypersensitive responses. We found eight WRR4A‐recognised CCGs and four WRR4B‐recognised CCGs, the first recognised proteins from A. candida for which the cognate immune receptors in A. thaliana are known. This multiple recognition capacity potentially explains the broad‐spectrum resistance to several A. candida races conferred by WRR4 paralogues. We further show that of five tested CCGs, three confer enhanced disease susceptibility when expressed in planta, consistent with A. candida CCG proteins being effectors.
Item Type: | Journal Article | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | S Agriculture > SB Plant culture | ||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||
Library of Congress Subject Headings (LCSH): | Albugo candida, Arabidopsis, Rust diseases, Plants -- Disease and pest resistance, Plant immunology, Oomycetes -- Genetics | ||||||||||||
Journal or Publication Title: | New Phytologist | ||||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||
ISSN: | 0028-646X | ||||||||||||
Official Date: | January 2023 | ||||||||||||
Dates: |
|
||||||||||||
Volume: | 237 | ||||||||||||
Number: | 2 | ||||||||||||
Page Range: | pp. 532-547 | ||||||||||||
DOI: | 10.1111/nph.18378 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 8 August 2022 | ||||||||||||
Date of first compliant Open Access: | 15 August 2022 | ||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year