The Library
Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures
Tools
Ding, Zhiyuan, Gao, Si, Fang, Weina, Huang, Chen, Zhou, Liqi, Pei, Xudong, Liu, Xiaoguo, Pan, Xiaoqing, Fan, Chunhai, Kirkland, Angus I. and Wang, Peng (2022) Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures. Nature Communications, 13 (1). 4787. doi:10.1038/s41467-022-32548-x ISSN 2041-1723.
|
PDF
WRAP-Three-dimensional-electron-ptychography-organic-inorganic-hybrid-nanostructures-22.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2482Kb) | Preview |
Official URL: https://doi.org/10.1038/s41467-022-32548-x
Abstract
Three dimensional scaffolded DNA origami with inorganic nanoparticles has been used to create tailored multidimensional nanostructures. However, the image contrast of DNA is poorer than those of the heavy nanoparticles in conventional transmission electron microscopy at high defocus so that the biological and non-biological components in 3D scaffolds cannot be simultaneously resolved using tomography of samples in a native state. We demonstrate the use of electron ptychography to recover high contrast phase information from all components in a DNA origami scaffold without staining. We further quantitatively evaluate the enhancement of contrast in comparison with conventional transmission electron microscopy. In addition, We show that for ptychography post-reconstruction focusing simplifies the workflow and reduces electron dose and beam damage.
Item Type: | Journal Article | |||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | T Technology > TP Chemical technology | |||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | |||||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Nanobiotechnology, DNA -- Industrial applications, Nanotechnology, DNA -- Structure, DNA -- Synthesis | |||||||||||||||||||||||||||
Journal or Publication Title: | Nature Communications | |||||||||||||||||||||||||||
Publisher: | Nature Publishing Group UK | |||||||||||||||||||||||||||
ISSN: | 2041-1723 | |||||||||||||||||||||||||||
Official Date: | 15 August 2022 | |||||||||||||||||||||||||||
Dates: |
|
|||||||||||||||||||||||||||
Volume: | 13 | |||||||||||||||||||||||||||
Number: | 1 | |||||||||||||||||||||||||||
Article Number: | 4787 | |||||||||||||||||||||||||||
DOI: | 10.1038/s41467-022-32548-x | |||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||
Reuse Statement (publisher, data, author rights): | ** From Springer Nature via Jisc Publications Router ** History: received 18-02-2021; accepted 04-08-2022; registration 05-08-2022; pub-electronic 15-08-2022; online 15-08-2022; collection 12-2022. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
Date of first compliant deposit: | 31 August 2022 | |||||||||||||||||||||||||||
Date of first compliant Open Access: | 31 August 2022 | |||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
|
|||||||||||||||||||||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year