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Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
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Qi, Linlin, Kwiatowski, Mateusz, Chen, Huihuang, Howrmayer, Lukas, Sinclair, Scott, Zou, Minxia, del Genio, Charo I., Kubeš, Martin F., Napier, Richard, Jaworski, Krzysztof and Friml, Jiří (2022) Adenylate cyclase activity of TIR1/AFB auxin receptors in plants. Nature (611). pp. 133-138. doi:10.1038/s41586-022-05369-7 ISSN 0028-0836.
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WRAP-denylate-cyclase-activity-TIR1-AFB-auxin-receptors-root-growth-22.pdf - Accepted Version - Requires a PDF viewer. Download (2948Kb) | Preview |
Official URL: https://doi.org/10.1038/s41586-022-05369-7
Abstract
The phytohormone auxin is the major coordinative signal in plant development1, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QK Botany | ||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Auxin, Plant cellular signal transduction, Plant hormones, Plants -- Development, Growth (Plants) -- Research | ||||||||||||
| Journal or Publication Title: | Nature | ||||||||||||
| Publisher: | Nature Publishing | ||||||||||||
| ISSN: | 0028-0836 | ||||||||||||
| Official Date: | 3 November 2022 | ||||||||||||
| Dates: |
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| Number: | 611 | ||||||||||||
| Page Range: | pp. 133-138 | ||||||||||||
| DOI: | 10.1038/s41586-022-05369-7 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Reuse Statement (publisher, data, author rights): | This version of the article has been accepted for publication, after peer review (when applicable) but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1038/s41586-022-05369-7. Use of this Accepted Version is subject to the publisher’s Accepted Manuscript terms of use https://www.springernature.com/gp/open-research/policies/acceptedmanuscript-terms. | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| Date of first compliant deposit: | 16 August 2022 | ||||||||||||
| Date of first compliant Open Access: | 20 March 2023 | ||||||||||||
| RIOXX Funder/Project Grant: |
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