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Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters
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Renne, Mike, Corey, Robin, Veríssimo Ferreira, Joana, Stansfeld, Phillip J. and Carvalho, Pedro (2022) Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters. Journal of Cell Biology, 221 (9). e202112068. doi:10.1083/jcb.202112068 ISSN 0021-9525.
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Official URL: http://dx.doi.org/10.1083/jcb.202112068
Abstract
Lipid droplets (LDs) are essential for cellular lipid homeostasis by storing diverse neutral lipids (NLs), such as triacylglycerol (TAG), steryl esters (SE), and retinyl esters (RE). A proper assembly of TAG-containing LDs at the ER requires Seipin, a conserved protein often mutated in lipodystrophies. Here, we show that the yeast Seipin Sei1 and its partner Ldb16 also promote the storage of other NL in LDs. Importantly, this role of Sei1/Ldb16 is evolutionarily conserved as expression of human-Seipin restored normal SE-containing LDs in yeast Seipin mutants. As in the case of TAG, the formation of SE-containing LDs requires interactions between hydroxyl-residues in human Seipin or yeast Ldb16 with NL carboxyl esters. These findings provide a universal mechanism for Seipin-mediated LD formation and suggest a model for how Seipin distinguishes NLs from aliphatic phospholipid acyl chains in the center of the membrane bilayer.
Item Type: | Journal Article | ||||||||||||||||||||||||
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Subjects: | Q Science > QP Physiology | ||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Lipids, Lipids -- Research, Membrane lipids, Lipids -- Metabolism | ||||||||||||||||||||||||
Journal or Publication Title: | Journal of Cell Biology | ||||||||||||||||||||||||
Publisher: | Rockefeller University Press | ||||||||||||||||||||||||
ISSN: | 0021-9525 | ||||||||||||||||||||||||
Book Title: | Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters | ||||||||||||||||||||||||
Official Date: | 8 August 2022 | ||||||||||||||||||||||||
Dates: |
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Volume: | 221 | ||||||||||||||||||||||||
Number: | 9 | ||||||||||||||||||||||||
Article Number: | e202112068 | ||||||||||||||||||||||||
DOI: | 10.1083/jcb.202112068 | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
Copyright Holders: | © 2022 Renne et al. | ||||||||||||||||||||||||
Date of first compliant deposit: | 18 August 2022 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 18 August 2022 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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