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Probing the effect of rigidity on the cellular uptake of core-shell nanoparticles : stiffness effects are size dependent
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Gurnani, Pratik, Sanchez-Cano, Carlos, Xandri-Monje, Helena, Zhang, Junliang, Ellacott, Sean H., Mansfield, Edward D. H., Hartlieb, Matthias, Dallmann, Robert and Perrier, Sébastien (2022) Probing the effect of rigidity on the cellular uptake of core-shell nanoparticles : stiffness effects are size dependent. Small, 18 (38). 2203070. doi:10.1002/smll.202203070 ISSN 1613-6810.
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WRAP-Probing-the-effect-of-rigidity-on-the-cellular-uptake-of-core-shell-nanoparticles-stiffness-effects-are-size-dependent-Dallmann-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3339Kb) | Preview |
Official URL: https://doi.org/10.1002/smll.202203070
Abstract
Nanoparticles are well established vectors for the delivery of a wide range of biomedically relevant cargoes. Numerous studies have investigated the impact of size, shape, charge, and surface functionality of nanoparticles on mammalian cellular uptake. Rigidity has been studied to a far lesser extent, and its effects are still unclear. Here, the importance of this property, and its interplay with particle size, is systematically explored using a library of core-shell spherical PEGylated nanoparticles synthesized by RAFT emulsion polymerization. Rigidity of these particles is controlled by altering the intrinsic glass transition temperature of their constituting polymers. Three polymeric core rigidities are tested: hard, medium, and soft using two particle sizes, 50 and 100 nm diameters. Cellular uptake studies indicate that softer particles are taken up faster and threefold more than harder nanoparticles with the larger 100 nm particles. In addition, the study indicates major differences in the cellular uptake pathway, with harder particles being internalized through clathrin- and caveolae-mediated endocytosis as well as macropinocytosis, while softer particles are taken up bycaveolae- and non-receptormediated endocytosis. However, 50 nm derivatives do not show any appreciable differences in uptake efficiency, suggesting that rigidity as a parameter in the biological regime may be size dependent.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | R Medicine > RS Pharmacy and materia medica | |||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Nanoparticles, Drug delivery systems, Polymers -- Experiments -- Research | |||||||||||||||||||||
| Journal or Publication Title: | Small | |||||||||||||||||||||
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | |||||||||||||||||||||
| ISSN: | 1613-6810 | |||||||||||||||||||||
| Official Date: | 22 September 2022 | |||||||||||||||||||||
| Dates: |
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| Volume: | 18 | |||||||||||||||||||||
| Number: | 38 | |||||||||||||||||||||
| Number of Pages: | 11 | |||||||||||||||||||||
| Article Number: | 2203070 | |||||||||||||||||||||
| DOI: | 10.1002/smll.202203070 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 22 August 2022 | |||||||||||||||||||||
| Date of first compliant Open Access: | 25 August 2022 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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