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Genetic screens identify additional genes implicated in envelope remodeling during the engulfment stage of Bacillus subtilis sporulation
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Chan, Helena, Taib, Najwa, Gilmore, Michael C., Mohamed, Ahmed M. T., Hanna, Kieran, Luhur, Johana, Nguyen, Hieu, Hafiz, Elham, Cava, Felipe, Gribaldo, Simonetta, Rudner, David and Rodrigues, Christopher D. A. (2022) Genetic screens identify additional genes implicated in envelope remodeling during the engulfment stage of Bacillus subtilis sporulation. mBio, 13 (5). e01732--22. doi:10.1128/mbio.01732-22 ISSN 2150-7511.
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Official URL: https://doi.org/10.1128/mbio.01732-22
Abstract
During bacterial endospore formation, the developing spore is internalized into the mother cell through a phagocytic-like process called engulfment, which involves synthesis and hydrolysis of peptidoglycan. Engulfment peptidoglycan hydrolysis requires the widely conserved and well-characterized DMP complex, composed of SpoIID, SpoIIM, and SpoIIP. In contrast, although peptidoglycan synthesis has been implicated in engulfment, the protein players involved are less well defined. The widely conserved SpoIIIAH-SpoIIQ interaction is also required for engulfment efficiency, functioning like a ratchet to promote membrane migration around the forespore. Here, we screened for additional factors required for engulfment using transposon sequencing in Bacillus subtilis mutants with mild engulfment defects. We discovered that YrvJ, a peptidoglycan hydrolase, and the MurA paralog MurAB, involved in peptidoglycan precursor synthesis, are required for efficient engulfment. Cytological analyses suggest that both factors are important for engulfment when the DMP complex is compromised and that MurAB is additionally required when the SpoIIIAH-SpoIIQ ratchet is abolished. Interestingly, despite the importance of MurAB for sporulation in B. subtilis, phylogenetic analyses of MurA paralogs indicate that there is no correlation between sporulation and the number of MurA paralogs and further reveal the existence of a third MurA paralog, MurAC, within the Firmicutes. Collectively, our studies identify two new factors that are required for efficient envelop remodeling during sporulation and highlight the importance of peptidoglycan precursor synthesis for efficient engulfment in B. subtilis and likely other endospore-forming bacteria.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QK Botany Q Science > QP Physiology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Bacillus subtilis -- Genetics, Bacterial genetics, Peptidoglycans , Bacterial cell walls, Morphogenesis , Spores | |||||||||||||||||||||
Journal or Publication Title: | mBio | |||||||||||||||||||||
Publisher: | American Society for Microbiology | |||||||||||||||||||||
ISSN: | 2150-7511 | |||||||||||||||||||||
Official Date: | 6 September 2022 | |||||||||||||||||||||
Dates: |
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Volume: | 13 | |||||||||||||||||||||
Number: | 5 | |||||||||||||||||||||
Page Range: | e01732--22 | |||||||||||||||||||||
DOI: | 10.1128/mbio.01732-22 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 1 September 2022 | |||||||||||||||||||||
Date of first compliant Open Access: | 9 September 2022 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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