The Library
Towards environmental detection of Chagas disease vectors and pathogen
Tools
Tools
Tools
Gysin, Grace, Urbano, Plutarco, Brandner-Garrod, Luke, Begum, Shahida, Kristan, Mojca, Walker, Thomas, Hernández, Carolina, Ramírez, Juan David and Messenger, Louisa A. (2022) Towards environmental detection of Chagas disease vectors and pathogen. Scientific Reports, 12 (1). 9849. doi:10.1038/s41598-022-14051-x ISSN 2045-2322.
|
PDF
s41598-022-14051-x.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2792Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/s41598-022-14051-x
Abstract
Chagas disease vector control relies on prompt, accurate identification of houses infested with triatomine bugs for targeted insecticide spraying. However, most current detection methods are laborious, lack standardization, have substantial operational costs and limited sensitivity, especially when triatomine bug densities are low or highly focal. We evaluated the use of FTA cards or cotton-tipped swabs to develop a low-technology, non-invasive method of detecting environmental DNA (eDNA) from both triatomine bugs and Trypanosoma cruzi for use in household surveillance in eastern Colombia, an endemic region for Chagas disease. Study findings demonstrated that Rhodnius prolixus eDNA, collected on FTA cards, can be detected at temperatures between 21 and 32 °C, when deposited by individual, recently blood-fed nymphs. Additionally, cotton-tipped swabs are a feasible tool for field sampling of both T. cruzi and R. prolixus eDNA in infested households and may be preferable due to their lower cost. eDNA detection should not yet replace current surveillance tools, but instead be evaluated in parallel as a more sensitive, higher-throughput, lower cost alternative. eDNA collection requires virtually no skills or resources in situ and therefore has the potential to be implemented in endemic communities as part of citizen science initiatives to control Chagas disease transmission.
| Item Type: | Journal Article | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QL Zoology R Medicine > RA Public aspects of medicine R Medicine > RC Internal medicine |
||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Chagas' disease , Chagas' disease -- Epidemiology , Chagas' disease -- Prevention, Conenoses as carriers of disease , Parasitic diseases | ||||||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||
| ISSN: | 2045-2322 | ||||||||||||
| Official Date: | 14 June 2022 | ||||||||||||
| Dates: |
|
||||||||||||
| Volume: | 12 | ||||||||||||
| Number: | 1 | ||||||||||||
| Article Number: | 9849 | ||||||||||||
| DOI: | 10.1038/s41598-022-14051-x | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
| Date of first compliant deposit: | 12 September 2022 | ||||||||||||
| Date of first compliant Open Access: | 12 September 2022 | ||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
