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Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events
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Mendieta-Serrano, Mario A., Dhar, Sunandan, Ng, Boon Heng, Narayanan, Rachna, Lee, Jorge J. Y., Ong, Hui Ting, Toh, Pearlyn Jia Ying, Röllin, Adrian, Roy, Sudipto and Saunders, Timothy E. (2022) Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events. Developmental Cell, 57 (17). pp. 2095-2110. doi:10.1016/j.devcel.2022.08.002 ISSN 1534-5807.
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WRAP-Slow-muscles-guide-fast-myocyte-fusion-to-ensure-robust-myotome-formation-despite-the-high-spatiotemporal-stochasticity-of-fusion-events-Saunders-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (32Mb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.devcel.2022.08.002
Abstract
Skeletal myogenesis is dynamic, and it involves cell-shape changes together with cell fusion and rearrangements. However, the final muscle arrangement is highly organized with striated fibers. By combining live imaging with quantitative analyses, we dissected fast-twitch myocyte fusion within the zebrafish myotome in toto. We found a strong mediolateral bias in fusion timing; however, at a cellular scale, there was heterogeneity in cell shape and the relationship between initial position of fast myocytes and resulting fusion partners. We show that the expression of the fusogen myomaker is permissive, but not instructive, in determining the spatiotemporal fusion pattern. Rather, we observed a close coordination between slow muscle rearrangements and fast myocyte fusion. In mutants that lack slow fibers, the spatiotemporal fusion pattern is substantially noisier. We propose a model in which slow muscles guide fast myocytes by funneling them close together, enhancing fusion probability. Thus, despite fusion being highly stochastic, a robust myotome structure emerges at the tissue scale.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QL Zoology | |||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Muscle cells , Myogenesis , Logperch , Skeleton -- Muscles -- Growth , Cell hybridization | |||||||||||||||
Journal or Publication Title: | Developmental Cell | |||||||||||||||
Publisher: | Cell Press | |||||||||||||||
ISSN: | 1534-5807 | |||||||||||||||
Official Date: | 12 September 2022 | |||||||||||||||
Dates: |
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Volume: | 57 | |||||||||||||||
Number: | 17 | |||||||||||||||
Page Range: | pp. 2095-2110 | |||||||||||||||
DOI: | 10.1016/j.devcel.2022.08.002 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 13 September 2022 | |||||||||||||||
Date of first compliant Open Access: | 13 September 2022 | |||||||||||||||
RIOXX Funder/Project Grant: |
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