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Detection of malaria sporozoites expelled during mosquito sugar feeding
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Brugman, V. A., Kristan, M., Gibbins, M. P., Angrisano, F., Sala, K. A., Dessens, J. T., Blagborough, A. M. and Walker, Thomas (2018) Detection of malaria sporozoites expelled during mosquito sugar feeding. Scientific Reports, 8 (1). 7545 . doi:10.1038/s41598-018-26010-6 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/s41598-018-26010-6
Abstract
Malaria is a severe disease of global importance transmitted by mosquitoes of the genus Anopheles. The ability to rapidly detect the presence of infectious mosquitoes able to transmit malaria is of vital importance for surveillance, control and elimination efforts. Current methods principally rely on large-scale mosquito collections followed by labour-intensive salivary gland dissections or enzyme-linked immunosorbent (ELISA) methods to detect sporozoites. Using forced salivation, we demonstrate here that Anopheles mosquitoes infected with Plasmodium expel sporozoites during sugar feeding. Expelled sporozoites can be detected on two sugar-soaked substrates, cotton wool and Whatman FTA cards, and sporozoite DNA is detectable using real-time PCR. These results demonstrate a simple and rapid methodology for detecting the presence of infectious mosquitoes with sporozoites and highlight potential laboratory applications for investigating mosquito-malaria interactions. Our results indicate that FTA cards could be used as a simple, effective and economical tool in enhancing field surveillance activities for malaria.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Scientific Reports | ||||||
Publisher: | Nature Publishing Group | ||||||
ISSN: | 2045-2322 | ||||||
Official Date: | 15 May 2018 | ||||||
Dates: |
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Volume: | 8 | ||||||
Number: | 1 | ||||||
Article Number: | 7545 | ||||||
DOI: | 10.1038/s41598-018-26010-6 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
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