The Library
Data for The first mitotic division of human embryos is highly error prone
Tools
Tools
Tools
Currie, Cerys E., Ford, Emma, Whyte, Lucy Benham, Taylor, Deborah M., Mihalas, Bettina P., Erent, Muriel, Marston, Adele L., Hartshorne, Geraldine M. and McAinsh, Andrew D. (2022) Data for The first mitotic division of human embryos is highly error prone. [Dataset]
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: https://omero.warwick.ac.uk/webclient/?show=projec...
Abstract
Aneuploidy in human embryos is surprisingly prevalent and increases drastically with maternal age, resulting in miscarriages, infertility and birth defects. Frequent errors during the meiotic divisions cause this aneuploidy, while age-independent errors during the first cleavage divisions of the embryo also contribute. However, the underlying mechanisms are poorly understood, largely because these events have never been visualised in living human embryos. Here, using cell-permeable DNA dyes, we film chromosome segregation during the first and second mitotic cleavage divisions in human embryos from women undergoing assisted reproduction following ovarian stimulation. We show that the first mitotic division takes several hours to complete and is highly variable. Timings of key mitotic events were, however, largely consistent with clinical videos of embryos that gave rise to live births. Multipolar divisions and lagging chromosomes during anaphase were frequent with no maternal age association. In contrast, the second mitosis was shorter and underwent mostly bipolar divisions with no detectable lagging chromosomes. We propose that the first mitotic division in humans is a unique and highly error-prone event, which contributes to fetal aneuploidies.
| Item Type: | Dataset | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history Q Science > QP Physiology R Medicine > RB Pathology |
|||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Mitosis, Aneuploidy, Human embryo | |||||||||||||||||||||
| Publisher: | University of Warwick, Warwick Medical School | |||||||||||||||||||||
| Official Date: | 21 September 2022 | |||||||||||||||||||||
| Dates: |
|
|||||||||||||||||||||
| Status: | Not Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Copyright Holders: | University of Warwick | |||||||||||||||||||||
| Description: | The dataset contains movies of human embryos consented to research progressing through the first two embryonic mitoses. The chromosomes have been visualised using SiR-DNA dye and imaged using a deltavision widefield microscope. Open access data is available at OMERO at Warwick: |
|||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
|||||||||||||||||||||
| Related URLs: | ||||||||||||||||||||||
| Contributors: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
