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Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities
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Schloss, Patrick D., Westcott, Sarah L., Ryabin, Thomas, Hall, Justine R., Hartmann, Martin, Hollister, Emily B., Lesniewski, Ryan A., Oakley, Brian B., Parks, Donovan H., Robinson, Courtney J., Sahl, Jason W., Stres, Blaz, Thallinger, Gerhard G., Van Horn, David J. and Weber, Carolyn F. (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, Vol.75 (No.23). pp. 7537-7541. doi:10.1128/AEM.01541-09 ISSN 0099-2240.
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Official URL: http://dx.doi.org/10.1128/AEM.01541-09
Abstract
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.
| Item Type: | Journal Article | ||||
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| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Applied and Environmental Microbiology | ||||
| Publisher: | American Society for Microbiology | ||||
| ISSN: | 0099-2240 | ||||
| Official Date: | 1 December 2009 | ||||
| Dates: |
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| Volume: | Vol.75 | ||||
| Number: | No.23 | ||||
| Number of Pages: | 5 | ||||
| Page Range: | pp. 7537-7541 | ||||
| DOI: | 10.1128/AEM.01541-09 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | College of Natural Resources and the Environment at the University of Massachusetts, Austrian GEN-AU project BIN, Sloan Foundation, National Science Foundation | ||||
| Grant number: | 0743432 |
Data sourced from Thomson Reuters' Web of Knowledge
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