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Incomplete citric acid cycle obliges aminolevulinic acid synthesis via the C5 pathway in a methylotroph
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Lloyd, Adrian J., Weitzman, P. D. and Soll, D. (1993) Incomplete citric acid cycle obliges aminolevulinic acid synthesis via the C5 pathway in a methylotroph. Journal of General Microbiology, 139 (12). pp. 2931-2938. doi:10.1099/00221287-139-12-2931 ISSN 0022-1287.
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Official URL: http://dx.doi.org/10.1099/00221287-139-12-2931
Abstract
The enzymic activities of the citric acid cycle and the connected pathway of 5-aminolevulinic acid (ALA) formation in the methylotroph Methylophilus rnethylotrophus (strain AS1) have been studied. The organism has the enzymes required for conversion of pyruvate to 2-oxoglutarate. Of these, isocitrate dehydrogenase is unusual because of its preference of NAD as coenzyme over NADP. In addition, the segment of the cycle that oxidizes 2-oxoglutarate to oxaloacetate is incomplete, lacking 2-oxoglutarate and succinate and malate dehydrogenase activities. Furthermore, alternative routes of 2-oxoglutarate oxidation to succinate are undetectable. The enzymes of the glyoxylate cycle are also absent. This suggests that the cycle in M. methylotrophus has no catabolic role and is purely biosynthetic. We also show that M. rnethylotrophus uses the C, pathway of ALA formation. Cell-free extracts can convert glutamate to ALA in an ATP-, NADPH- and tRNA-dependent manner via the intermediate
formation of Glu-tRNAG'" and glutamate 1-semialdehyde. Consistent with the absence of a detectable route by which it could synthesize succinate, M. rnethylotrophus cannot generate ALA from succinyl-CoA and glycine, the pathway found in mammalian cells and yeast.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Journal or Publication Title: | Journal of General Microbiology | ||||||||
| Publisher: | Society for General Microbiology | ||||||||
| ISSN: | 0022-1287 | ||||||||
| Official Date: | 1 December 1993 | ||||||||
| Dates: |
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| Volume: | 139 | ||||||||
| Number: | 12 | ||||||||
| Page Range: | pp. 2931-2938 | ||||||||
| DOI: | 10.1099/00221287-139-12-2931 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) |
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