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Refinement of Haemophilus influenzae diaminopimelic acid epimerase (DapF) at 1.75 A resolution suggests a mechanism for stereocontrol during catalysis
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Lloyd, Adrian J., Huyton, Trevor, Turkenburg, Johan and Roper, David I. (2004) Refinement of Haemophilus influenzae diaminopimelic acid epimerase (DapF) at 1.75 A resolution suggests a mechanism for stereocontrol during catalysis. Acta crystallographica. Section D, Biological crystallography, 60 (2). pp. 397-400. doi:10.1107/S0907444903027999
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Lloyd et al (2004) Acta Cryst. D60, 397-400.pdf - Published Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (478Kb) |
Official URL: https://doi.org/10.1107/S0907444903027999
Abstract
Diaminopimelate (DAP) epimerase (DapF) is central to the biosynthesis of both lysine and cell-wall peptidoglycan in many bacteria species. The peptidoglycan layer provides great potential for the development of novel antimicrobials as it is a uniquely prokaryotic feature. Crystals of recombinant Haemophilus influenzae DapF that diffract to beyond 2 A resolution have been obtained which facilitated the solution of the structure by molecular replacement at a resolution approximately 1 A higher than that previously determined. An analysis of the structure (i) in comparison to other PLP-independent racemaces and (ii) in relation to the catalytic mechanism and stereospecificity of DapF is presented.
Item Type: | Journal Item | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | Acta crystallographica. Section D, Biological crystallography | ||||||||
Publisher: | Wiley | ||||||||
ISSN: | 0907-4449 | ||||||||
Official Date: | February 2004 | ||||||||
Dates: |
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Volume: | 60 | ||||||||
Number: | 2 | ||||||||
Page Range: | pp. 397-400 | ||||||||
DOI: | 10.1107/S0907444903027999 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 3 October 2022 |
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