Rapid endocytosis provides restricted somatic expression of a K+ channel in central neurons
Corrêa, Sônia A. L., Mueller, Juergen, Collingridge, Graham L. and Marrion, Neil V.. (2009) Rapid endocytosis provides restricted somatic expression of a K+ channel in central neurons. Journal of Cell Science, Vol.122 (No.22). pp. 4186-4194. ISSN 0021-9533Full text not available from this repository.
Official URL: http://dx.doi.org/10.1242/jcs.058420
Trafficking motifs present in the intracellular regions of ion channels affect their subcellular location within neurons. The mechanisms that control trafficking to dendrites of central neurons have been identified, but it is not fully understood how channels are localized to the soma. We have now identified a motif within the calcium-activated potassium channel K(Ca)2.1 (SK1) that results in somatic localization. Transfection of hippocampal neurons with K(Ca)2.1 subunits causes expression of functional channels in only the soma and proximal processes. By contrast, expressed K(Ca)2.3 subunits are located throughout the processes of transfected neurons. Point mutation of K(Ca)2.1 within this novel motif to mimic a sequence present in the C-terminus of K(Ca)2.3 causes expression of K(Ca)2.1 subunits throughout the processes. We also demonstrate that blocking of clathrin-mediated endocytosis causes K(Ca)2.1 subunit expression to mimic that of the mutated subunit. The role of this novel motif is therefore not to directly target trafficking of the channel to subcellular compartments, but to regulate channel location by subjecting it to rapid clathrin-mediated endocytosis.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Medicine > Warwick Medical School
|Journal or Publication Title:||Journal of Cell Science|
|Publisher:||The Company of Biologists Ltd.|
|Official Date:||15 November 2009|
|Number of Pages:||9|
|Page Range:||pp. 4186-4194|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Medical Research Council (UK)|
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