The Library
Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate
Tools
Tools
Tools
Ijaz, Umer Zeeshan, Gundogdu, Ozan, Keating, Ciara, Eekert, Miriam van, Gibson, Walter, Parkhill, Julian, Abilahi, Faraji, Liseki, Benard, Nguyen, Viet-Anh, Sudgen, Steven, Quince, Christopher , Ensink, Jeroen H. J., Torondel, Belen and Walker, Alan W. (2022) Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate. Frontiers in Microbiology, 13 . 960747. doi:10.3389/fmicb.2022.960747
|
PDF
WRAP-Analysis-of-pit-latrine-microbiota-reveals-depth-related-variation-in-composition-and-key-parameters-and-taxa-associated-with-latrine-fill-up-rate-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2500Kb) | Preview |
Official URL: https://doi.org/10.3389/fmicb.2022.960747
Abstract
Pit latrines are used by billions of people globally, often in developing countries where they provide a low-tech and low-cost sanitation method. However, health and social problems can arise from a lack of emptying or maintenance of these facilities. A better understanding of the biological and environmental parameters within pit latrines could inform attempts to enhance material decomposition rates, and therefore slow fill-up rate. In this study, we have performed a spatial analysis of 35 Tanzanian pit latrines to identify bacteria and environmental factors that are associated with faster or slower pit latrine fill-up rates. Using ordination of microbial community data, we observed a linear gradient in terms of beta diversity with increasing pit latrine sample depth, corresponding to a shift in microbial community structure from gut-associated families in the top layer to environmental- and wastewater-associated taxa at greater depths. We also investigated the bacteria and environmental parameters associated with fill-up rates, and identified pH, volatile solids, and volatile fatty acids as features strongly positively correlated with pit latrine fill-up rates, whereas phosphate was strongly negatively correlated with fill-up rate. A number of pit latrine microbiota taxa were also correlated with fill-up rates. Using a multivariate regression, we identified the Lactobacillaceae and Incertae_Sedis_XIII taxa as particularly strongly positively and negatively correlated with fill-up rate, respectively. This study therefore increases knowledge of the microbiota within pit latrines, and identifies potentially important bacteria and environmental variables associated with fill-up rates. These new insights may be useful for future studies investigating the decomposition process within pit latrines.
| Item Type: | Journal Article | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RA Public aspects of medicine |
||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Sanitation, Outhouses, Toilets, Microbiology, Biodegradation | ||||||||||||||||||||||||
| Journal or Publication Title: | Frontiers in Microbiology | ||||||||||||||||||||||||
| Publisher: | Frontiers Research Foundation | ||||||||||||||||||||||||
| ISSN: | 1664-302X | ||||||||||||||||||||||||
| Official Date: | 23 September 2022 | ||||||||||||||||||||||||
| Dates: |
|
||||||||||||||||||||||||
| Volume: | 13 | ||||||||||||||||||||||||
| Number of Pages: | 14 | ||||||||||||||||||||||||
| Article Number: | 960747 | ||||||||||||||||||||||||
| DOI: | 10.3389/fmicb.2022.960747 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
