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Label-free quantitative proteomic analysis of adult Drosophila heads
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Zhang, Yan and Nezis, Ioannis P. (2022) Label-free quantitative proteomic analysis of adult Drosophila heads. STAR Protocols, 3 (4). 101830. doi:10.1016/j.xpro.2022.101830 ISSN 2666-1667.
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Official URL: https://doi.org/10.1016/j.xpro.2022.101830
Abstract
LIR motif-containing proteins (LIRCPs) bind to LDS (LIR motif docking site) of Atg8-family proteins. In this protocol, we describe steps to identify Drosophila LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins from adult Drosophila heads, followed by liquid chromatography–mass spectrometry (LC-MS/MS) analysis. We also describe screening steps of upregulated proteins in Atg8a LDS mutants, leading to identification of novel LIRCPs in Drosophila.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | Cytology, Drosophila -- Genetics, Proteomics -- Experiments -- Methodology | |||||||||
Journal or Publication Title: | STAR Protocols | |||||||||
Publisher: | Cell Press | |||||||||
ISSN: | 2666-1667 | |||||||||
Official Date: | 16 December 2022 | |||||||||
Dates: |
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Volume: | 3 | |||||||||
Number: | 4 | |||||||||
Article Number: | 101830 | |||||||||
DOI: | 10.1016/j.xpro.2022.101830 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 13 October 2022 | |||||||||
Date of first compliant Open Access: | 25 November 2022 | |||||||||
RIOXX Funder/Project Grant: |
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